Probing cytoskeletal mechanics using biochemical inhibitors

Kenneth M. Pryse, Teresa M. Abney, Guy M. Genin, Elliot L. Elson

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Quantifying the mechanics of the cytoskeletons of living cells is important for understanding several physiologic and pathologic cellular functions, such as wound healing and cellular migration in cancer. Our laboratory develops three-dimensional tissue constructs for assaying cytoskeletal mechanics in controlled conditions. These tissue constructs consist of defined components such as chick embryo fibroblasts and reconstituted rat tail collagen; fibroblasts remodel the collagen extracellular matrix (ECM) and develop a structural environment representative of that which would exist in a natural tissue. Our protocol for quantifying the microscale mechanics of the proteins that comprise the cytoskeleton involves mechanical testing of a tissue construct first in a bath that contains nutrition medium to support the active physiologic functioning of the cells, and next in the presence of inhibitors that selectively eliminate specific cytoskeletal structures. By solving an inverse homogenization problem, the mechanical functioning of these proteins at the cellular level can be estimated. Here, we present a combination of mechanical testing and imaging results to quantify the effects of specific inhibitors on cytoskeletal and extracellular matrix form and function.

Original languageEnglish
Title of host publicationASME 2010 Summer Bioengineering Conference, SBC 2010
Pages357-358
Number of pages2
EditionPARTS A AND B
DOIs
StatePublished - Dec 1 2010
EventASME 2010 Summer Bioengineering Conference, SBC 2010 - Naples, FL, United States
Duration: Jun 16 2010Jun 19 2010

Publication series

NameASME 2010 Summer Bioengineering Conference, SBC 2010
NumberPARTS A AND B

Conference

ConferenceASME 2010 Summer Bioengineering Conference, SBC 2010
Country/TerritoryUnited States
CityNaples, FL
Period06/16/1006/19/10

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