Probable antibody-mediated failure of two sequential ABO-compatible hepatic allografts in a single recipient

L. E. Ratner, D. Phelan, E. M. Brunt, T. Mohanakumar, D. W. Hanto

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


Two sequential ABO-compatible orthotopic liver allografts failed, despite excellent initial posttransplant function, in a patient with preformed donor- specific alloantibodies. There was no evidence of cell-mediated rejection. Retrospective crossmatching of recipient serum, obtained immediately prior to the first transplant, revealed the presence of lymphocytotoxic antibodies directed against donor class I HLA B17, at a titer of greater than 1:32,768. Similarly, lymphocytotoxic antibodies directed against the second donor's class I HLA A2 phenotype were detected on retrospective crossmatching utilizing both the three-wash Amos technique (TWA-CDC), and the anti-human immunoglobulin augmented technique (AHG-CDC), at a titer of greater than 1:32,768. Anti-class I specific alloantibodies were eluted from both failed liver grafts at titers of 1:256. The hepatic necrosis in zones 3 and 2 that were observed on histologic examination, and the profound refractory consumptive thrombocytopenia subsequent to each transplant may have been the result of antibody-mediated rejection by preformed lymphocytotoxic antibodies. Despite the liver's remarkable capacity to withstand antibody- mediated injury, primary humoral rejection following ABO compatible liver transplantation may occur if extremely high titers of preformed allospecific lymphocytotoxic antibodies are present.

Original languageEnglish
Pages (from-to)814-819
Number of pages6
Issue number4
StatePublished - 1993


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