PRMT5 Protects Genomic Integrity during Global DNA Demethylation in Primordial Germ Cells and Preimplantation Embryos

Shinseog Kim, Ufuk Günesdogan, Jan J. Zylicz, Jamie A. Hackett, Delphine Cougot, Siqin Bao, Caroline Lee, Sabine Dietmann, George E. Allen, Roopsha Sengupta, M. Azim Surani

Research output: Contribution to journalArticle

Abstract

Primordial germ cells (PGCs) and preimplantation embryos undergo epigenetic reprogramming, which includes comprehensive DNA demethylation. We found that PRMT5, an arginine methyltransferase, translocates from the cytoplasm to the nucleus during this process. Here we show that conditional loss of PRMT5 in early PGCs causes complete male and female sterility, preceded by the upregulation of LINE1 and IAP transposons as well as activationofa DNA damage response. Similarly, loss of maternal-zygotic PRMT5 also leads to IAP upregulation. PRMT5 is necessary for the repressive H2A/H4R3me2s chromatin modification on LINE1 and IAP transposons in PGCs, directly implicating this modification in transposon silencing during DNA hypomethylation. PRMT5 translocates back to the cytoplasm subsequently, to participate in the previously described PIWI-interacting RNA (piRNA) pathway that promotes transposon silencing via de novo DNA remethylation. Thus, PRMT5 is directly involved in genome defense during preimplantation development and in PGCs at the time of global DNA demethylation.

Original languageEnglish
Pages (from-to)564-579
Number of pages16
JournalMolecular cell
Volume56
Issue number4
DOIs
StatePublished - Nov 20 2014
Externally publishedYes

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    Kim, S., Günesdogan, U., Zylicz, J. J., Hackett, J. A., Cougot, D., Bao, S., Lee, C., Dietmann, S., Allen, G. E., Sengupta, R., & Surani, M. A. (2014). PRMT5 Protects Genomic Integrity during Global DNA Demethylation in Primordial Germ Cells and Preimplantation Embryos. Molecular cell, 56(4), 564-579. https://doi.org/10.1016/j.molcel.2014.10.003