TY - JOUR
T1 - PrimerBank
T2 - A resource of human and mouse PCR primer pairs for gene expression detection and quantification
AU - Spandidos, Athanasia
AU - Wang, Xiaowei
AU - Wang, Huajun
AU - Seed, Brian
PY - 2009/11/10
Y1 - 2009/11/10
N2 - PrimerBank (http://pga.mgh.harvard.edu/primerbank/) is a public resource for the retrieval of human and mouse primer pairs for gene expression analysis by PCR and Quantitative PCR (QPCR). A total of 306 800 primers covering most known human and mouse genes can be accessed from the PrimerBank database, together with information on these primers such as Tm, location on the transcript and amplicon size. For each gene, at least one primer pair has been designed and in many cases alternative primer pairs exist. Primers have been designed to work under the same PCR conditions, thus facilitating high-throughput QPCR. There are several ways to search for primers for the gene(s) of interest, such as by: GenBank accession number, NCBI protein accession number, NCBI gene ID, PrimerBank ID, NCBI gene symbol or gene description (keyword). In all, 26 855 primer pairs covering most known mouse genes have been experimentally validated by QPCR, agarose gel analysis, sequencing and BLAST, and all validation data can be freely accessed from the PrimerBank web site.
AB - PrimerBank (http://pga.mgh.harvard.edu/primerbank/) is a public resource for the retrieval of human and mouse primer pairs for gene expression analysis by PCR and Quantitative PCR (QPCR). A total of 306 800 primers covering most known human and mouse genes can be accessed from the PrimerBank database, together with information on these primers such as Tm, location on the transcript and amplicon size. For each gene, at least one primer pair has been designed and in many cases alternative primer pairs exist. Primers have been designed to work under the same PCR conditions, thus facilitating high-throughput QPCR. There are several ways to search for primers for the gene(s) of interest, such as by: GenBank accession number, NCBI protein accession number, NCBI gene ID, PrimerBank ID, NCBI gene symbol or gene description (keyword). In all, 26 855 primer pairs covering most known mouse genes have been experimentally validated by QPCR, agarose gel analysis, sequencing and BLAST, and all validation data can be freely accessed from the PrimerBank web site.
UR - http://www.scopus.com/inward/record.url?scp=75549089019&partnerID=8YFLogxK
U2 - 10.1093/nar/gkp1005
DO - 10.1093/nar/gkp1005
M3 - Article
C2 - 19906719
AN - SCOPUS:75549089019
SN - 0305-1048
VL - 38
SP - D792-D799
JO - Nucleic acids research
JF - Nucleic acids research
IS - SUPPL.1
M1 - gkp1005
ER -