Primary murine growth plate and articular chondrocyte isolation and cell culture

Jennifer H. Jonason, Donna Hoak, Regis J. O'Keefe

Research output: Chapter in Book/Report/Conference proceedingChapter

12 Scopus citations

Abstract

The ability to isolate primary chondrocytes from wild-type and genetically altered mice has provided tremendous advances in the understanding of signaling networks that regulate chondrocytes in health and disease. Isolation of chondrocytes from both growth plate tissues and articular cartilage has been challenging due to the cells being embedded within a highly organized tissue matrix. Here we describe highly reproducible methods for the isolation of pure populations of growth plate chondrocytes from the murine sternum and ribs and articular chondrocytes from the knee joint.

Original languageEnglish
Title of host publicationOsteoporosis and Osteoarthritis
PublisherSpringer New York
Pages11-18
Number of pages8
ISBN (Electronic)9781493916191
ISBN (Print)9781493916184
DOIs
StatePublished - Oct 20 2014
Externally publishedYes

Keywords

  • Articular cartilage
  • Articular chondrocyte
  • Cell culture
  • Cell isolation
  • Growth plate cartilage
  • Growth plate chondrocyte
  • Tissue digestion

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    Jonason, J. H., Hoak, D., & O'Keefe, R. J. (2014). Primary murine growth plate and articular chondrocyte isolation and cell culture. In Osteoporosis and Osteoarthritis (pp. 11-18). Springer New York. https://doi.org/10.1007/978-1-4939-1619-1_2