TY - JOUR
T1 - Prevention of the graft-versus-host reaction in newborn mice by antibodies to tumor necrosis factor-alpha
AU - Shalaby, M. Refaat
AU - Fendly, Brian
AU - Sheehan, Kathleen C.
AU - Schreiber, Robert D.
AU - Ammann, Arthur J.
PY - 1989/6
Y1 - 1989/6
N2 - The influence of antibodies to recombinant murine tumor necrosis factor-alpha (anti—rMuTNF-α) on the development of the graft-versus-host reaction in vivo was investigated. This was done by evaluating the degree of splenomegaly in newborn BDF1 (B6×DBA/2) mice 10–11 days after injection of autologous BDF1 (controls) or semiallogeneic B6 (test) spleen cells. Splenomegaly, as reflected by the spleen index, among test BDF1 mice was 3–4-fold greater than the SI of control BDF1 mice. However, the treatment of test BDF1 mice with multiple injections of rabbit anti-rMuTNF-α anti-serum resulted in a significant reduction in the SIs. In additional experiments, hamster monoclonal antibodies to rMuTNF-α were also shown to be effective in preventing the GVHR in vivo. Neither normal rabbit serum nor normal hamster IgG affected the GVHR in test BDF1 mice. These results indicate that TNF-α plays an important role in the development of the GVHR in vivo and suggest that antibodies, or other antagonists, to TNF-α may have potential use for the management of organ or tissue transplants.
AB - The influence of antibodies to recombinant murine tumor necrosis factor-alpha (anti—rMuTNF-α) on the development of the graft-versus-host reaction in vivo was investigated. This was done by evaluating the degree of splenomegaly in newborn BDF1 (B6×DBA/2) mice 10–11 days after injection of autologous BDF1 (controls) or semiallogeneic B6 (test) spleen cells. Splenomegaly, as reflected by the spleen index, among test BDF1 mice was 3–4-fold greater than the SI of control BDF1 mice. However, the treatment of test BDF1 mice with multiple injections of rabbit anti-rMuTNF-α anti-serum resulted in a significant reduction in the SIs. In additional experiments, hamster monoclonal antibodies to rMuTNF-α were also shown to be effective in preventing the GVHR in vivo. Neither normal rabbit serum nor normal hamster IgG affected the GVHR in test BDF1 mice. These results indicate that TNF-α plays an important role in the development of the GVHR in vivo and suggest that antibodies, or other antagonists, to TNF-α may have potential use for the management of organ or tissue transplants.
UR - http://www.scopus.com/inward/record.url?scp=0024412123&partnerID=8YFLogxK
U2 - 10.1097/00007890-198906000-00028
DO - 10.1097/00007890-198906000-00028
M3 - Article
C2 - 2525289
AN - SCOPUS:0024412123
SN - 0041-1337
VL - 47
SP - 1057
EP - 1061
JO - Transplantation
JF - Transplantation
IS - 6
ER -