TY - JOUR
T1 - Prevalence of antiviral drug resistance in untreated patients with cytomegalovirus retinitis
AU - Liu, Weiqun
AU - Shum, Carol
AU - Martin, Daniel F.
AU - Kuppermann, Baruch D.
AU - Hall, Anthony J.H.
AU - Margolis, Todd P.
N1 - Funding Information:
Financial support: Fight for Sight research division of Prevent Blindness America, Research to Prevent Blindness, Roche Pharmaceuticals(CYT054), and Alfred Hospital Senior Medical Staff Traveling Scholarship (A.J.H.H.).
PY - 2000
Y1 - 2000
N2 - The purpose of this study was to determine the prevalence of UL97 resistance mutations in cytomegalovirus (CMV) DNA amplified from the eyes of patients with AIDS and newly diagnosed CMV retinitis. Relevant segments of the CMV UL97 gene were amplified from vitreous humor, after which restriction digest screening was performed for resistance mutations at codons 460, 520, 591, 592, 594, 595, and 603. Mutations were confirmed by DNA sequencing. Vitreous from 21 eyes with AIDS-related non-CMV viral retinitis served as negative controls. CMV DNA was successfully amplified from 195 of 204 eyes. A resistance mutation was found in only a single eye, a TG mutation at base 1774, predicting a cysteine to glycine mutation at codon 592. The prevalence of UL97 resistance mutations in the eyes of patients with newly diagnosed CMV retinitis is very low.
AB - The purpose of this study was to determine the prevalence of UL97 resistance mutations in cytomegalovirus (CMV) DNA amplified from the eyes of patients with AIDS and newly diagnosed CMV retinitis. Relevant segments of the CMV UL97 gene were amplified from vitreous humor, after which restriction digest screening was performed for resistance mutations at codons 460, 520, 591, 592, 594, 595, and 603. Mutations were confirmed by DNA sequencing. Vitreous from 21 eyes with AIDS-related non-CMV viral retinitis served as negative controls. CMV DNA was successfully amplified from 195 of 204 eyes. A resistance mutation was found in only a single eye, a TG mutation at base 1774, predicting a cysteine to glycine mutation at codon 592. The prevalence of UL97 resistance mutations in the eyes of patients with newly diagnosed CMV retinitis is very low.
UR - http://www.scopus.com/inward/record.url?scp=0033796102&partnerID=8YFLogxK
U2 - 10.1086/315851
DO - 10.1086/315851
M3 - Article
C2 - 10979924
AN - SCOPUS:0033796102
SN - 0022-1899
VL - 182
SP - 1234
EP - 1238
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 4
ER -