TY - JOUR
T1 - Presentation of Candida albicans and purified protein derivative soluble antigens by Epstein-Barr virus-transformed human lymphoblastoid B-cell lines
AU - Reitnauer, Pamela J.
AU - Brown, Lawrence R.
AU - Hank, Jacquelyn A.
AU - Sondel, Paul M.
N1 - Funding Information:
’ This work was supported by Grants CH-237A of the American Cancer Society and ROI-CA32685-03 of the National Institutes of Health. This is Paper 2753 from the Laboratory of Genetics. * Supported in part by the University of Wisconsin Department of Genetics Predoctoral Training Grant GM 07133. ’ Scholar of the Leukemia Society of America and a G. A. and J. L. Hartford Foundation Fellow. 4 Abbreviations used: APC, antigen-presenting cells; cGy, centi-Gray; CAN, Candida albicans; EBV- LCL, Epstein-Barr virus-transformed B lymphoblastoid cell lines; Hepes, 4-(2-hydroxylethyl)-1-piperaxine-ethanesulfonic acid, HS-RPMI, human serum-supplemented tissue culture medium; MHC, major histocompatibility complex; MLC, mixed-lymphocyte culture; PBL, peripheral blood lymphocytes; PLT, primed lymphocyte test; PPD, purified protein derivative of tuberculin; TCGF, T-cell growth factor; [‘H]TdR, [‘HImethyl-labeled tbymidine; PHA, phytohemagglutinin; MoAb, monoclonal antibodies; TT, tetanus toxoid antigen; IL-1, interleukin-1.
PY - 1986/4/15
Y1 - 1986/4/15
N2 - Cells other than the macrophage can function as antigen-presenting cells (APCs). These class II-bearing accessory cells include dendritic cells, epidermal Langerhans cells, B cells, murine B-cell tumors, and human Epstein-Barr virus-transformed lymphoblastoid cell lines (EBV-LCL). We investigated the ability of EBV-LCL to present two soluble antigens, Candida albicans and purified protein derivative of tuberculin (PPD). The EBV-LCL derived from B cells of two different individuals can present both antigens to bulk cultures of autologous antigen-primed peripheral blood lymphocytes. The responses of PPD-reactive T-cell clones were weaker to PPD when presented by EBV-LCL than by PBL-APCs, with some clones responding only to PPD presented by PBL-APCs. This suggests that EBV-LCL are not equivalent to PEL monocytes in APC function, and that expression of class II major histocompatibility complex antigen is not sufficient in enabling antigen-presenting capability.
AB - Cells other than the macrophage can function as antigen-presenting cells (APCs). These class II-bearing accessory cells include dendritic cells, epidermal Langerhans cells, B cells, murine B-cell tumors, and human Epstein-Barr virus-transformed lymphoblastoid cell lines (EBV-LCL). We investigated the ability of EBV-LCL to present two soluble antigens, Candida albicans and purified protein derivative of tuberculin (PPD). The EBV-LCL derived from B cells of two different individuals can present both antigens to bulk cultures of autologous antigen-primed peripheral blood lymphocytes. The responses of PPD-reactive T-cell clones were weaker to PPD when presented by EBV-LCL than by PBL-APCs, with some clones responding only to PPD presented by PBL-APCs. This suggests that EBV-LCL are not equivalent to PEL monocytes in APC function, and that expression of class II major histocompatibility complex antigen is not sufficient in enabling antigen-presenting capability.
UR - http://www.scopus.com/inward/record.url?scp=0022654325&partnerID=8YFLogxK
U2 - 10.1016/0008-8749(86)90228-5
DO - 10.1016/0008-8749(86)90228-5
M3 - Article
C2 - 3019572
AN - SCOPUS:0022654325
SN - 0008-8749
VL - 99
SP - 196
EP - 208
JO - Cellular Immunology
JF - Cellular Immunology
IS - 1
ER -