TY - JOUR
T1 - Preparation, purification and properties of a crosslinked trimer of G-actin
AU - Gilbert, Helen R.
AU - Frieden, Carl
N1 - Funding Information:
Acknowledgement: This work has been supported by Grant
PY - 1983/3/16
Y1 - 1983/3/16
N2 - Phenylenebismaleimide has been used to form crosslinks between actin monomers [Knight, P. and Offer, G. (1978) Biochem. J. 175, 1023-1032]. We have purified a trimer of actin monomers as well as a dimer and a mixture of higher molecular weight oligomers. The trimer is much more effective than the dimer in enhancing the rate of polymerization while higher oligomers do not appear to be any more effective than the trimer. A lag in the polymerization process, as measured fluorescence enhancement of trace pyrene-actin, still occurs in the presence of trimers serving as the nuclei, suggesting that the mechanism for polymerization is more complex than nucleation followed by elongation.
AB - Phenylenebismaleimide has been used to form crosslinks between actin monomers [Knight, P. and Offer, G. (1978) Biochem. J. 175, 1023-1032]. We have purified a trimer of actin monomers as well as a dimer and a mixture of higher molecular weight oligomers. The trimer is much more effective than the dimer in enhancing the rate of polymerization while higher oligomers do not appear to be any more effective than the trimer. A lag in the polymerization process, as measured fluorescence enhancement of trace pyrene-actin, still occurs in the presence of trimers serving as the nuclei, suggesting that the mechanism for polymerization is more complex than nucleation followed by elongation.
UR - http://www.scopus.com/inward/record.url?scp=0021105819&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(83)90320-0
DO - 10.1016/0006-291X(83)90320-0
M3 - Article
C2 - 6838567
AN - SCOPUS:0021105819
SN - 0006-291X
VL - 111
SP - 404
EP - 408
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -