Preparation of stable single-chain trimers engineered with peptide, β2 microglobulin, and MHC heavy chain

Ted Hansen, Y. Y.Lawrence Yu, Daved H. Fremont

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

This unit describes a method for constructing a class I MHC molecule with a bound peptide as a single polypeptide chain, termed SCT, for single chain trimer. The component organization of the SCT appears to be widely applicable to different mouse or human MHC class I isotypes bound by different antigenic peptides. The enhanced peptide occupancy afforded by the SCT format makes these molecules effective reagents as DNA vaccines, multimeric staining reagents to enumerate CD8 T cells, and probes of lymphocyte biology.

Original languageEnglish
Pages (from-to)17.5.1-17.5.17
JournalCurrent Protocols in Immunology
Issue numberSUPPL. 87
DOIs
StatePublished - Dec 1 2009

Keywords

  • Lymphocyte development
  • Recombinant MHC/peptide complexes
  • Tetramers
  • Vaccines

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