Abstract
This unit describes a method for constructing a class I MHC molecule with a bound peptide as a single polypeptide chain, termed SCT, for single chain trimer. The component organization of the SCT appears to be widely applicable to different mouse or human MHC class I isotypes bound by different antigenic peptides. The enhanced peptide occupancy afforded by the SCT format makes these molecules effective reagents as DNA vaccines, multimeric staining reagents to enumerate CD8 T cells, and probes of lymphocyte biology.
Original language | English |
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Pages (from-to) | 17.5.1-17.5.17 |
Journal | Current Protocols in Immunology |
Issue number | SUPPL. 87 |
DOIs | |
State | Published - 2009 |
Keywords
- Lymphocyte development
- Recombinant MHC/peptide complexes
- Tetramers
- Vaccines