Abstract
This unit describes a method for constructing a class I MHC molecule with a bound peptide as a single polypeptide chain, termed SCT, for single chain trimer. The component organization of the SCT appears to be widely applicable to different mouse or human MHC class I isotypes bound by different antigenic peptides. The enhanced peptide occupancy afforded by the SCT format makes these molecules effective reagents as DNA vaccines, multimeric staining reagents to enumerate CD8 T cells, and probes of lymphocyte biology.
| Original language | English |
|---|---|
| Pages (from-to) | 17.5.1-17.5.17 |
| Journal | Current Protocols in Immunology |
| Issue number | SUPPL. 87 |
| DOIs | |
| State | Published - Dec 1 2009 |
Keywords
- Lymphocyte development
- Recombinant MHC/peptide complexes
- Tetramers
- Vaccines
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Hansen, T., Yu, Y. Y. L., & Fremont, D. H. (2009). Preparation of stable single-chain trimers engineered with peptide, β2 microglobulin, and MHC heavy chain. Current Protocols in Immunology, (SUPPL. 87), 17.5.1-17.5.17. https://doi.org/10.1002/0471142735.im1705s87