Preparation of Small Temporal Bones for High-Resolution Light Microscopy

Richard A. Chole, George L. Charpied

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


A technique was used for rapid processing of small temporal bones for high-resolution light microscopy. Temporal bones were decalcified in edetic acid and embedded in glycol methacrylate. Sections (thickness, 1 μm) were stained with a variety of stains that demonstrated cellular details not usually seen with conventional methods. This technique is suitable for routine histologic, histochemical, and radioautographic studies of small temporal bones.

Original languageEnglish
Pages (from-to)610-612
Number of pages3
JournalArchives of Otolaryngology
Issue number10
StatePublished - Oct 1981


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