TY - JOUR
T1 - Preliminary Observations on the Spatial Correlation Between Short-Burst Microbubble Oscillations and Vascular Bioeffects
AU - Chen, Hong
AU - Brayman, Andrew A.
AU - Evan, Andrew P.
AU - Matula, Thomas J.
N1 - Funding Information:
The authors thank Drs. Lawrence A. Crum, Michael R. Bailey, Wayne Kreider, Joo Ha Hwang, Oleg A. Sapozhnikov and Yak-Nam Wang for many helpful discussions. They also thank Frank Starr, Francis Olson, Brian MacConaghy and Stephanie Lara for assistance with the experiments. This work was supported in part by NIH grants EB000350 (NIBIB), AR053652 (NIAMS) and P01DK043881 (NIDDKD).
PY - 2012/12
Y1 - 2012/12
N2 - The objective of this preliminary study was to examine the spatial correlation between microbubble (MB)-induced vessel wall displacements and resultant microvascular bioeffects. MBs were injected into venules in ex vivo rat mesenteries and insonated by a single short ultrasound pulse with a center frequency of 1 MHz and peak negative pressures spanning the range of 1.5-5.6 MPa. MB and vessel dynamics were observed under ultra-high speed photomicrography. The tissue was examined by histology or transmission electron microscopy for vascular bioeffects. Image registration allowed for spatial correlation of MB-induced vessel wall motion to corresponding vascular bioeffects, if any. In cases in which damage was observed, the vessel wall had been pulled inward by more than 50% of the its initial radius. The observed damage was characterized by the separation of the endothelium from the vessel wall. Although the study is limited to a small number of observations, analytic statistical results suggest that vessel invagination comprises a principal mechanism for bioeffects in venules by microbubbles.
AB - The objective of this preliminary study was to examine the spatial correlation between microbubble (MB)-induced vessel wall displacements and resultant microvascular bioeffects. MBs were injected into venules in ex vivo rat mesenteries and insonated by a single short ultrasound pulse with a center frequency of 1 MHz and peak negative pressures spanning the range of 1.5-5.6 MPa. MB and vessel dynamics were observed under ultra-high speed photomicrography. The tissue was examined by histology or transmission electron microscopy for vascular bioeffects. Image registration allowed for spatial correlation of MB-induced vessel wall motion to corresponding vascular bioeffects, if any. In cases in which damage was observed, the vessel wall had been pulled inward by more than 50% of the its initial radius. The observed damage was characterized by the separation of the endothelium from the vessel wall. Although the study is limited to a small number of observations, analytic statistical results suggest that vessel invagination comprises a principal mechanism for bioeffects in venules by microbubbles.
KW - High speed photomicrography
KW - Microbubble dynamics
KW - Microvessels
KW - Ultrasound bioeffects
KW - Ultrasound contrast agents
KW - Ultrasound-induced vascular damage
UR - http://www.scopus.com/inward/record.url?scp=84870293287&partnerID=8YFLogxK
U2 - 10.1016/j.ultrasmedbio.2012.08.014
DO - 10.1016/j.ultrasmedbio.2012.08.014
M3 - Article
C2 - 23069136
AN - SCOPUS:84870293287
SN - 0301-5629
VL - 38
SP - 2151
EP - 2162
JO - Ultrasound in Medicine and Biology
JF - Ultrasound in Medicine and Biology
IS - 12
ER -