TY - JOUR
T1 - Preferential human eosinophil chemotactic activity of the platelet-activating factor (PAF) 1-0-hexadecyl-2-acetyl-sn-glyceryl-3-phosphocholine (AGEPC)
AU - Sigal, C. Elliott
AU - Valone, Frank H.
AU - Holtzman, Michael J.
AU - Goetzl, Edward J.
PY - 1987/3
Y1 - 1987/3
N2 - The chemotactic responses of human blood neutrophils and of eosinophils of two different densities, which were resolved by centrifugation on gradients of polyvinylpyrrolidone-coated silica gel (Percoll), were quantified in modified Boyden micropore filter chambers using highly purified synthetic 1-0-hexadecyl-2-acetyl-sn-glyceryl-3-phosphocholine (AGEPC or PAFacether) and leukotriene B4 (LTB4) as stimuli. Maximal chemotactic responses of the densest eosinophils, less dense eosinophils, and neutrophils were evoked by 1 n M, 100 n M, and 1 μM PAFacether, respectively, and by 30-100, 30-100, and 10 n M LTB4. The magnitude of the maximal chemotactic response to PAFacether of the densest eosinophils was significantly greater than that of neutrophils. The eosinophil responses to PAFacether were chemotactic, as distinguished from chemokinetic, and were not influenced by the percentage of contaminating neutrophils. PAFacether is a more potent chemotactic factor for eosinophils than neutrophils and selectively attracts the densest population of human blood eosinophils.
AB - The chemotactic responses of human blood neutrophils and of eosinophils of two different densities, which were resolved by centrifugation on gradients of polyvinylpyrrolidone-coated silica gel (Percoll), were quantified in modified Boyden micropore filter chambers using highly purified synthetic 1-0-hexadecyl-2-acetyl-sn-glyceryl-3-phosphocholine (AGEPC or PAFacether) and leukotriene B4 (LTB4) as stimuli. Maximal chemotactic responses of the densest eosinophils, less dense eosinophils, and neutrophils were evoked by 1 n M, 100 n M, and 1 μM PAFacether, respectively, and by 30-100, 30-100, and 10 n M LTB4. The magnitude of the maximal chemotactic response to PAFacether of the densest eosinophils was significantly greater than that of neutrophils. The eosinophil responses to PAFacether were chemotactic, as distinguished from chemokinetic, and were not influenced by the percentage of contaminating neutrophils. PAFacether is a more potent chemotactic factor for eosinophils than neutrophils and selectively attracts the densest population of human blood eosinophils.
KW - Eosinophil
KW - allergy
KW - chemotaxis
KW - platelet-activating factor (PAF)
UR - http://www.scopus.com/inward/record.url?scp=0023108387&partnerID=8YFLogxK
U2 - 10.1007/BF00916012
DO - 10.1007/BF00916012
M3 - Article
C2 - 3571436
AN - SCOPUS:0023108387
SN - 0271-9142
VL - 7
SP - 179
EP - 184
JO - Journal of Clinical Immunology
JF - Journal of Clinical Immunology
IS - 2
ER -