TY - JOUR
T1 - Precision editing of the gut microbiota ameliorates colitis
AU - Zhu, Wenhan
AU - Winter, Maria G.
AU - Byndloss, Mariana X.
AU - Spiga, Luisella
AU - Duerkop, Breck A.
AU - Hughes, Elizabeth R.
AU - Büttner, Lisa
AU - De Lima Romão, Everton
AU - Behrendt, Cassie L.
AU - Lopez, Christopher A.
AU - Sifuentes-Dominguez, Luis
AU - Huff-Hardy, Kayci
AU - Wilson, R. Paul
AU - Gillis, Caroline C.
AU - Tükel, Çagla
AU - Koh, Andrew Y.
AU - Burstein, Ezra
AU - Hooper, Lora V.
AU - Bäumler, Andreas J.
AU - Winter, Sebastian E.
N1 - Publisher Copyright:
© 2018 Macmillan Publishers Limited, part of Springer Nature. All rights reserved.
PY - 2018/1/11
Y1 - 2018/1/11
N2 - Inflammatory diseases of the gastrointestinal tract are frequently associated with dysbiosis, characterized by changes in gut microbial communities that include an expansion of facultative anaerobic bacteria of the Enterobacteriaceae family (phylum Proteobacteria). Here we show that a dysbiotic expansion of Enterobacteriaceae during gut inflammation could be prevented by tungstate treatment, which selectively inhibited molybdenum-cofactor-dependent microbial respiratory pathways that are operational only during episodes of inflammation. By contrast, we found that tungstate treatment caused minimal changes in the microbiota composition under homeostatic conditions. Notably, tungstate-mediated microbiota editing reduced the severity of intestinal inflammation in mouse models of colitis. We conclude that precision editing of the microbiota composition by tungstate treatment ameliorates the adverse effects of dysbiosis in the inflamed gut.
AB - Inflammatory diseases of the gastrointestinal tract are frequently associated with dysbiosis, characterized by changes in gut microbial communities that include an expansion of facultative anaerobic bacteria of the Enterobacteriaceae family (phylum Proteobacteria). Here we show that a dysbiotic expansion of Enterobacteriaceae during gut inflammation could be prevented by tungstate treatment, which selectively inhibited molybdenum-cofactor-dependent microbial respiratory pathways that are operational only during episodes of inflammation. By contrast, we found that tungstate treatment caused minimal changes in the microbiota composition under homeostatic conditions. Notably, tungstate-mediated microbiota editing reduced the severity of intestinal inflammation in mouse models of colitis. We conclude that precision editing of the microbiota composition by tungstate treatment ameliorates the adverse effects of dysbiosis in the inflamed gut.
UR - https://www.scopus.com/pages/publications/85040444802
U2 - 10.1038/nature25172
DO - 10.1038/nature25172
M3 - Article
C2 - 29323293
AN - SCOPUS:85040444802
SN - 0028-0836
VL - 553
SP - 208
EP - 211
JO - Nature
JF - Nature
IS - 7687
ER -