@article{8bed93fd1d3e4374a96aa90907ac468c,
title = "Precise Tuning of Cortical Contractility Regulates Cell Shape during Cytokinesis",
abstract = "Taneja et al. describe distinct roles for the two myosin-II paralogs in regulating actin cortex mechanics during cell division. Myosin-IIA generates cortex tension, while myosin-IIB maintains cortical stability. Optimal levels of the two paralogs within hetero-filaments at the cortex are required for shape stability and cytokinetic fidelity during cell division.",
keywords = "actin cortex, binucleation, bleb, cell division, cortex tension, cytokinesis, hydrostatic pressure, myosin IIA, myosin IIB, spindle",
author = "Nilay Taneja and Bersi, {Matthew R.} and Baillargeon, {Sophie M.} and Fenix, {Aidan M.} and Cooper, {James A.} and Ryoma Ohi and Vivian Gama and Merryman, {W. David} and Burnette, {Dylan T.}",
note = "Funding Information: We thank David Miller for providing glass micro-capillaries for the indentation assay; Miguel Vincente-Manzanares for providing MII chimeras; Nikon Center of Excellence, Vanderbilt University for access to the Nikon Spinning Disk microscope and technical support; Kristopher Burkewitz for lending his InjectMan for micro-pipette aspiration; and the Vanderbilt Mass Spectrometry Core Lab for assistance with quantification of paralog levels in HAP1 cells. This work was funded by a Maximizing Investigators' Research Award (MIRA) from the National Institute of General Medical Sciences (NIGMS) (R35 GM125028-01 to D.T.B.); an American Heart Association Predoctoral Fellowship (18PRE33960551 to N.T.); a MIRA (R35-GM128915 to V.G.); and a MIRA (R35-HL135790 to W.D.M. and R01-GM086610 to R.O.). D.T.B. and N.T. designed experiments. N.T. S.M.B. and A.M.F. performed experiments. N.T. M.R.B. A.M.F. and J.A.C. analyzed the data. M.R.B. and W.D.M. helped in the interpretation of cell indentation experiments. R.O. helped in experiment design. V.G. provided access to reagents (human H9 ESC cell line). N.T. and D.T.B. wrote the manuscript. All authors read and commented on the manuscript. The authors declare no competing interests. Funding Information: We thank David Miller for providing glass micro-capillaries for the indentation assay; Miguel Vincente-Manzanares for providing MII chimeras; Nikon Center of Excellence, Vanderbilt University for access to the Nikon Spinning Disk microscope and technical support; Kristopher Burkewitz for lending his InjectMan for micro-pipette aspiration; and the Vanderbilt Mass Spectrometry Core Lab for assistance with quantification of paralog levels in HAP1 cells. This work was funded by a Maximizing Investigators' Research Award (MIRA) from the National Institute of General Medical Sciences ( NIGMS ) ( R35 GM125028-01 to D.T.B.); an American Heart Association Predoctoral Fellowship ( 18PRE33960551 to N.T.); a MIRA ( R35-GM128915 to V.G.); and a MIRA ( R35-HL135790 to W.D.M. and R01-GM086610 to R.O.). Publisher Copyright: {\textcopyright} 2020 The Author(s)",
year = "2020",
month = apr,
day = "7",
doi = "10.1016/j.celrep.2020.03.041",
language = "English",
volume = "31",
journal = "Cell Reports",
issn = "2211-1247",
number = "1",
}