Practical aspects of mating and tetrad analysis

Susan K. Dutcher

doi:10.1016/B978-0-12-822457-1.00009-1

Practical aspects of mating and tetrad analysis

Susan K. Dutcher

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

1 Scopus citations

Abstract

The meiotic analysis of mutants is a cornerstone of genetic analysis. Meiotic analysis has been used since the work of T. H. Morgan in Drosophila and provides a key tool for biological studies. This chapter describes the experimental protocols used in Chlamydomonas for mating and the subsequent analysis of meiotic products using tetrads and random progeny analysis. The use of two other stages of the life cycle, dikaryons and vegetative diploids, is described in Vol. 3 Chapter 1.

Original language	English
Title of host publication	The Chlamydomonas Sourcebook
Subtitle of host publication	Volume 1: Introduction to Chlamydomonas and Its Laboratory Use
Publisher	Elsevier
Pages	315-323
Number of pages	9
Volume	1
ISBN (Electronic)	9780128224571
ISBN (Print)	9780128224588
DOIs	https://doi.org/10.1016/B978-0-12-822457-1.00009-1
State	Published - Jan 1 2023

Keywords

Chlamydomonas
dikaryons
mating
tetrads

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10.1016/B978-0-12-822457-1.00009-1

Cite this

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AB - The meiotic analysis of mutants is a cornerstone of genetic analysis. Meiotic analysis has been used since the work of T. H. Morgan in Drosophila and provides a key tool for biological studies. This chapter describes the experimental protocols used in Chlamydomonas for mating and the subsequent analysis of meiotic products using tetrads and random progeny analysis. The use of two other stages of the life cycle, dikaryons and vegetative diploids, is described in Vol. 3 Chapter 1.

KW - Chlamydomonas

KW - dikaryons

KW - mating

KW - tetrads

UR - https://www.scopus.com/pages/publications/85160169419

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M3 - Chapter

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VL - 1

SP - 315

EP - 323

BT - The Chlamydomonas Sourcebook

PB - Elsevier

ER -

Practical aspects of mating and tetrad analysis

Abstract

Keywords

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