Potassium Flux through Gramicidin Ion Channels Is Augmented in Vesicles Comprised of Plasmenylcholine: Correlations between Gramicidin Conformation and Function in Chemically Distinct Host Bilayer Matrices

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Abstract

The functional role of distinct phospholipid subclasses and molecular species in modulating gramicidin-mediated K+ flux was characterized through quantification of changes in the fluorescence intensity of ion specific fluorescent probes encapsulated inside vesicles comprised of individual molecular species pf plasmenylcholine and phosphatidylcholine. The rate constant of gramicidin-mediated K+ ion flux across bilayers comprised of l-G-(Z)-hexadec-T-enyl-2-octadec-9'-enoyl-sn-glycero-3-phosphocholine (plasmenylcholine) was 18.9 ± 1.7 s-1, while that present across bilayers comprised of 1-hexadecanoyl- 2-octadec-9'-enoyl-sn-glycero-3-phosphocholine (phosphatidylcholine) was 12.3 ± 1.5 s-1. The observed changes were not due to alterations in the nature of the sn-2 aliphatic chain or the net surface charge present at the membrane interface and were unaltered by the addition of several amphiphilic agents (including charged amphiphiles), suggesting that the observed alterations specifically reflect changes in channel function which result from the covalent alteration of host phospholipid in the proximal portion of the in-1 aliphatic chain (i.e., phospholipid subclass-specific alterations). Addition of cholesterol to bilayer matrices comprised of plasmenylcholine resulted in dose-dependent attentuation of the rate of gramicidin-mediated K+ flux, but did not alter the rate of gramicidin-mediated K+ flux in membranes comprised of phosphatidylcholine. Gramicidin ion channels experience distinct environments in membranes comprised of phosphatidylcholine and plasmenylcholine host lipids demonstrated by both the different fluorescence anisotropies of endogenous tryptophan residues and the different C=O stretching frequencies of intramonomer carbonyls in gramicidin incorporated into these two choline glycerophospholipid subclasses. Collectively, these results demonstrate the importance of the aggregate properties of hostguest complexes comprised of ion channels and phospholipids in biologic membranes as a primary determinant of ion channel function and identify a likely mechanism contributing to the predominance of plasmalogen molecular species in electrically active membranes.

Original languageEnglish
Pages (from-to)7356-7364
Number of pages9
JournalBiochemistry
Volume34
Issue number22
DOIs
StatePublished - Jun 1995

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