TY - JOUR
T1 - Postfusional control of quantal current shape
AU - Pawlu, Christian
AU - DiAntonio, Aaron
AU - Heckmann, Manfred
N1 - Funding Information:
We thank M. Hammel for expert technical assistance; A. Roth for advice during the initial stages of the simulations; H. Bellen for Drosophila endophilin null mutants; T.L. Schwarz for the synaptotagmin mutants; and J. Behrends, J. Eilers, S. Hallermann, P. Jonas, S. Sigrist, and G. Stuart for their comments on the manuscript. A.D. was supported by a Burroughs-Wellcome Career Award. The work in Germany was supported by the Deutsche Forschungsgemeinschaft.
PY - 2004/5/27
Y1 - 2004/5/27
N2 - Whether glutamate is released rapidly, in an all-or-none manner, or more slowly, in a regulated manner, is a matter of debate. We analyzed the time course of excitatory postsynaptic currents (EPSCs) at glutamatergic neuromuscular junctions of Drosophila and found that the decay phase of EPSCs was protracted to a variable extent. The protraction was more pronounced in evoked and spontaneous quantal EPSCs than in action potential-evoked multiquantal EPSCs; reduced in quantal EPSCs from endophilin null mutants, which maintain release via kiss-and-run; and dependent on synaptotagmin isoform, calcium, and protein phosphorylation. Our data indicate that glutamate is released from individual synaptic vesicles for milliseconds through a fusion pore. Quantal glutamate discharge time course depends on presynaptic calcium inflow and the molecular composition of the release machinery.
AB - Whether glutamate is released rapidly, in an all-or-none manner, or more slowly, in a regulated manner, is a matter of debate. We analyzed the time course of excitatory postsynaptic currents (EPSCs) at glutamatergic neuromuscular junctions of Drosophila and found that the decay phase of EPSCs was protracted to a variable extent. The protraction was more pronounced in evoked and spontaneous quantal EPSCs than in action potential-evoked multiquantal EPSCs; reduced in quantal EPSCs from endophilin null mutants, which maintain release via kiss-and-run; and dependent on synaptotagmin isoform, calcium, and protein phosphorylation. Our data indicate that glutamate is released from individual synaptic vesicles for milliseconds through a fusion pore. Quantal glutamate discharge time course depends on presynaptic calcium inflow and the molecular composition of the release machinery.
UR - http://www.scopus.com/inward/record.url?scp=2442542273&partnerID=8YFLogxK
U2 - 10.1016/S0896-6273(04)00269-7
DO - 10.1016/S0896-6273(04)00269-7
M3 - Article
C2 - 15157422
AN - SCOPUS:2442542273
SN - 0896-6273
VL - 42
SP - 607
EP - 618
JO - Neuron
JF - Neuron
IS - 4
ER -