Polymorphic secreted kinases are key virulence factors in toxoplasmosis

J. P.J. Saeij; J. P. Boyle; S. Coller; S. Taylor; L. D. Sibley; E. T. Brooke-Powell; J. W. Ajioka; J. C. Boothroyd

doi:10.1126/science.1133690

Polymorphic secreted kinases are key virulence factors in toxoplasmosis

J. P.J. Saeij, J. P. Boyle, S. Coller, S. Taylor, L. D. Sibley, E. T. Brooke-Powell, J. W. Ajioka, J. C. Boothroyd

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Abstract

The majority of known Toxoplasma gondii isolates from Europe and North America belong to three clonal lines that differ dramatically in their virulence, depending on the host. To identify the responsible genes, we mapped virulence in F₁ progeny derived from crosses between type II and type III strains, which we introduced into mice. Five virulence (VIR) loci were thus identified, and for two of these, genetic complementation showed that a predicted protein kinase (ROP18 and ROP16, respectively) is the key molecule. Both are hypervariable rhoptry proteins that are secreted into the host cell upon invasion. These results suggest that secreted kinases unique to the Apicomplexa are crucial in the host-pathogen interaction.

Original language	English
Pages (from-to)	1780-1783
Number of pages	4
Journal	Science
Volume	314
Issue number	5806
DOIs	https://doi.org/10.1126/science.1133690
State	Published - Dec 15 2006

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title = "Polymorphic secreted kinases are key virulence factors in toxoplasmosis",

abstract = "The majority of known Toxoplasma gondii isolates from Europe and North America belong to three clonal lines that differ dramatically in their virulence, depending on the host. To identify the responsible genes, we mapped virulence in F1 progeny derived from crosses between type II and type III strains, which we introduced into mice. Five virulence (VIR) loci were thus identified, and for two of these, genetic complementation showed that a predicted protein kinase (ROP18 and ROP16, respectively) is the key molecule. Both are hypervariable rhoptry proteins that are secreted into the host cell upon invasion. These results suggest that secreted kinases unique to the Apicomplexa are crucial in the host-pathogen interaction.",

author = "Saeij, {J. P.J.} and Boyle, {J. P.} and S. Coller and S. Taylor and Sibley, {L. D.} and Brooke-Powell, {E. T.} and Ajioka, {J. W.} and Boothroyd, {J. C.}",

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AU - Saeij, J. P.J.

AU - Boyle, J. P.

AU - Coller, S.

AU - Taylor, S.

AU - Sibley, L. D.

AU - Brooke-Powell, E. T.

AU - Ajioka, J. W.

AU - Boothroyd, J. C.

PY - 2006/12/15

Y1 - 2006/12/15

N2 - The majority of known Toxoplasma gondii isolates from Europe and North America belong to three clonal lines that differ dramatically in their virulence, depending on the host. To identify the responsible genes, we mapped virulence in F1 progeny derived from crosses between type II and type III strains, which we introduced into mice. Five virulence (VIR) loci were thus identified, and for two of these, genetic complementation showed that a predicted protein kinase (ROP18 and ROP16, respectively) is the key molecule. Both are hypervariable rhoptry proteins that are secreted into the host cell upon invasion. These results suggest that secreted kinases unique to the Apicomplexa are crucial in the host-pathogen interaction.

AB - The majority of known Toxoplasma gondii isolates from Europe and North America belong to three clonal lines that differ dramatically in their virulence, depending on the host. To identify the responsible genes, we mapped virulence in F1 progeny derived from crosses between type II and type III strains, which we introduced into mice. Five virulence (VIR) loci were thus identified, and for two of these, genetic complementation showed that a predicted protein kinase (ROP18 and ROP16, respectively) is the key molecule. Both are hypervariable rhoptry proteins that are secreted into the host cell upon invasion. These results suggest that secreted kinases unique to the Apicomplexa are crucial in the host-pathogen interaction.

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VL - 314

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Polymorphic secreted kinases are key virulence factors in toxoplasmosis

Abstract

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