TY - JOUR
T1 - Polydopamine-Mesoporous Silica Core-Shell Nanoparticles for Combined Photothermal Immunotherapy
AU - Seth, Anushree
AU - Gholami Derami, Hamed
AU - Gupta, Prashant
AU - Wang, Zheyu
AU - Rathi, Priya
AU - Gupta, Rohit
AU - Cao, Thao
AU - Morrissey, Jeremiah J.
AU - Singamaneni, Srikanth
N1 - Funding Information:
We acknowledge support from National Institutes of Health (R21 CA236652 and R01 CA141521). The authors would like to thank Dr. Delaram Shakiba for helping with confocal fluorescence microscopy, Prof. Jai Rudra for providing access to a flow cytometer, and the Nano Research Facility (NRF) and Institute of Materials Science and Engineering (IMSE) at the Washington University for providing access to electron microscopy facilities.
Funding Information:
We acknowledge support from National Institutes of Health (R21 CA236652 and R01 CA141521). The authors would like to thank Dr. Delaram Shakiba for helping with confocal fluorescence microscopy Prof. Jai Rudra for providing access to a flow cytometer, and the Nano Research Facility (NRF) and Institute of Materials Science and Engineering (IMSE) at the Washington University for providing access to electron microscopy facilities.
Publisher Copyright:
Copyright © 2020 American Chemical Society.
PY - 2020/9/23
Y1 - 2020/9/23
N2 - Cancer immunotherapy involves a cascade of events that ultimately leads to cytotoxic immune cells effectively identifying and destroying cancer cells. Responsive nanomaterials, which enable spatiotemporal orchestration of various immunological events for mounting a highly potent and long-lasting antitumor immune response, are an attractive platform to overcome challenges associated with existing cancer immunotherapies. Here, we report a multifunctional near-infrared (NIR)-responsive core-shell nanoparticle, which enables (i) photothermal ablation of cancer cells for generating tumor-associated antigen (TAA) and (ii) triggered release of an immunomodulatory drug (gardiquimod) for starting a series of immunological events. The core of these nanostructures is composed of a polydopamine nanoparticle, which serves as a photothermal agent, and the shell is made of mesoporous silica, which serves as a drug carrier. We employed a phase-change material as a gatekeeper to achieve concurrent release of both TAA and adjuvant, thus efficiently activating the antigen-presenting cells. Photothermal immunotherapy enabled by these nanostructures resulted in regression of primary tumor and significantly improved inhibition of secondary tumor in a mouse melanoma model. These biocompatible, biodegradable, and NIR-responsive core-shell nanostructures simultaneously deliver payload and cause photothermal ablation of the cancer cells. Our results demonstrate potential of responsive nanomaterials in generating highly synergistic photothermal immunotherapeutic response.
AB - Cancer immunotherapy involves a cascade of events that ultimately leads to cytotoxic immune cells effectively identifying and destroying cancer cells. Responsive nanomaterials, which enable spatiotemporal orchestration of various immunological events for mounting a highly potent and long-lasting antitumor immune response, are an attractive platform to overcome challenges associated with existing cancer immunotherapies. Here, we report a multifunctional near-infrared (NIR)-responsive core-shell nanoparticle, which enables (i) photothermal ablation of cancer cells for generating tumor-associated antigen (TAA) and (ii) triggered release of an immunomodulatory drug (gardiquimod) for starting a series of immunological events. The core of these nanostructures is composed of a polydopamine nanoparticle, which serves as a photothermal agent, and the shell is made of mesoporous silica, which serves as a drug carrier. We employed a phase-change material as a gatekeeper to achieve concurrent release of both TAA and adjuvant, thus efficiently activating the antigen-presenting cells. Photothermal immunotherapy enabled by these nanostructures resulted in regression of primary tumor and significantly improved inhibition of secondary tumor in a mouse melanoma model. These biocompatible, biodegradable, and NIR-responsive core-shell nanostructures simultaneously deliver payload and cause photothermal ablation of the cancer cells. Our results demonstrate potential of responsive nanomaterials in generating highly synergistic photothermal immunotherapeutic response.
KW - NIR-responsive drug delivery
KW - cancer immunotherapy
KW - mesoporous silica
KW - photothermal therapy
KW - polydopamine nanoparticles
UR - http://www.scopus.com/inward/record.url?scp=85091565472&partnerID=8YFLogxK
U2 - 10.1021/acsami.0c10781
DO - 10.1021/acsami.0c10781
M3 - Article
C2 - 32838525
AN - SCOPUS:85091565472
VL - 12
SP - 42499
EP - 42510
JO - ACS Applied Materials and Interfaces
JF - ACS Applied Materials and Interfaces
SN - 1944-8244
IS - 38
ER -