TY - JOUR
T1 - Podosome expression in osteoclasts
T2 - influence of high extracellular calcium concentration.
AU - Teti, A.
AU - Barattolo, R.
AU - Grano, M.
AU - Colucci, S.
AU - Argentino, L.
AU - Teitelbaum, S. L.
AU - Hruska, K. A.
AU - Santacroce, G.
AU - Zambonin Zallone, A.
PY - 1989/11
Y1 - 1989/11
N2 - In this study the effect of high extracellular calcium concentration has been evaluated, by immunofluorescence, on podosome expression in chicken osteoclasts. Cells were cultured in presence of 0.2 and 4 mM calcium for 90 minutes and microfilaments were detected, after fixation and permeabilization, by decoration with rodhamine conjugated phalloidin. Results showed that increased extracellular calcium concentration induces the inhibition of podosome expression indicating that these close-contact areas are capable of calcium-mediated regulation.
AB - In this study the effect of high extracellular calcium concentration has been evaluated, by immunofluorescence, on podosome expression in chicken osteoclasts. Cells were cultured in presence of 0.2 and 4 mM calcium for 90 minutes and microfilaments were detected, after fixation and permeabilization, by decoration with rodhamine conjugated phalloidin. Results showed that increased extracellular calcium concentration induces the inhibition of podosome expression indicating that these close-contact areas are capable of calcium-mediated regulation.
UR - http://www.scopus.com/inward/record.url?scp=0024757125&partnerID=8YFLogxK
M3 - Article
C2 - 2629823
AN - SCOPUS:0024757125
SN - 0037-8771
VL - 65
SP - 1039
EP - 1043
JO - Bollettino della Societa italiana di biologia sperimentale
JF - Bollettino della Societa italiana di biologia sperimentale
IS - 11
ER -