Pn-AMP1, a plant defense protein, induces actin depolarization in yeasts

Choon Koo Ja, Boyoung Lee, Michael E. Young, Chul Koo Sung, John A. Cooper, Dongwon Baek, Oh Lim Chae, Yeol Lee Sang, Dae Jin Yun, Je Cho Moo

Research output: Contribution to journalArticlepeer-review

50 Scopus citations

Abstract

Pn-AMP1, Pharbitis nil antimicrobial peptide 1, is a small cysteine-rich peptide implicated in host-plant defense. We show here that Pn-AMP1 causes depolarization of the actin cytoskeleton in Saccharomyces cerevisiae and Candida albicans. Pn-AMP1 induces rapid depolarization of actin cables and patches within 15 min. Increased osmolarity or temperature induces transient actin depolarization and results in increased sensitivity to Pn-AMP1, while cells conditioned to these stresses show less sensitivity. Mutations in components of a cell wall integrity pathway (Wsc1p, Rom2p, Bck1p and Mpk1p), which regulate actin repolarization, result in increased sensitivity to Pn-AMP1. A genetic screen reveals that mutations in components of the α-1,6- mannosyltransferase complex (Mnn10p, Mnn11p and Och1p), which regulate mannosylation of cell wall proteins, confer resistance to Pn-AMP1. FITC-conjugated Pn-AMP1 localizes to the outer surface of the cell with no significant staining observed in spheroplasts. Taken together, these results indicate that cell wall proteins are determinants of resistance to Pn-AMP1, and the ability of a plant defense protein to induce actin depolarization is important for its antifungal activity.

Original languageEnglish
Pages (from-to)1669-1680
Number of pages12
JournalPlant and Cell Physiology
Volume45
Issue number11
DOIs
StatePublished - Nov 2004

Keywords

  • Actin cytoskeleton
  • Cell wall integrity pathway
  • Hevein-like peptide
  • Plant antifungal protein
  • Yeast

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