TY - JOUR
T1 - Platelet-activating factor and Escherichia coli O157:H7 infections
AU - Smith, Jodi M.
AU - Jones, Falaah
AU - Ciol, Marcia A.
AU - Jelacic, Srdjan
AU - Boster, Daniel R.
AU - Watkins, Sandra L.
AU - Williams, Glyn D.
AU - Tarr, Phillip I.
AU - Henderson, William R.
PY - 2002
Y1 - 2002
N2 - The role of platelet-activating factor (PAF), a phospholipid inflammatory mediator, in Escherichia coli O157:H7-associated hemolytic uremic syndrome (HUS) is unknown. PAF is synthesized by diverse cells and is degraded by PAF-acetylhydrolase (PAF-AH). Deficient PAF-AH activity results from a G→T transversion at position 994 of exon 9. We examined children infected with E. coli O157:H7 to determine if PAF levels or the PAF-AH (G994T) mutation reflects the risk of developing HUS. Plasma PAF concentrations were determined using chloroform/methanol extraction, thin layer chromatography purification, and scintillation proximity assay in 10 patients with uncomplicated infectLon (UI), 10 infected patients who subsequently developed HUS (pre-HUS), 5 HUS patients, and 8 healthy controls. The PAF-AH (G994T) allele frequency was determined in 52 UI children, 15 with HUS, and 11 controls. Wilcoxon rank sum tests were performed to test differences in location (median) of pairs of groups. PAF levels were higher in the UI (P=0.04) and pre-HUS (P=0.01) groups than in healthy controls. No subject had the PAF-AH (G994T) allele. Thus, elevated plasma PAF levels occur in E. coli O157:H7 -infected children, even without HUS, but diminish when HUS develops. The PAF-AH (G994T) allele does not contribute to the risk of developing HUS.
AB - The role of platelet-activating factor (PAF), a phospholipid inflammatory mediator, in Escherichia coli O157:H7-associated hemolytic uremic syndrome (HUS) is unknown. PAF is synthesized by diverse cells and is degraded by PAF-acetylhydrolase (PAF-AH). Deficient PAF-AH activity results from a G→T transversion at position 994 of exon 9. We examined children infected with E. coli O157:H7 to determine if PAF levels or the PAF-AH (G994T) mutation reflects the risk of developing HUS. Plasma PAF concentrations were determined using chloroform/methanol extraction, thin layer chromatography purification, and scintillation proximity assay in 10 patients with uncomplicated infectLon (UI), 10 infected patients who subsequently developed HUS (pre-HUS), 5 HUS patients, and 8 healthy controls. The PAF-AH (G994T) allele frequency was determined in 52 UI children, 15 with HUS, and 11 controls. Wilcoxon rank sum tests were performed to test differences in location (median) of pairs of groups. PAF levels were higher in the UI (P=0.04) and pre-HUS (P=0.01) groups than in healthy controls. No subject had the PAF-AH (G994T) allele. Thus, elevated plasma PAF levels occur in E. coli O157:H7 -infected children, even without HUS, but diminish when HUS develops. The PAF-AH (G994T) allele does not contribute to the risk of developing HUS.
KW - Hemolytic uremic syndrome
KW - Platelet-activating factor
KW - Platelet-activating factor-acetylhydrolase
UR - http://www.scopus.com/inward/record.url?scp=12244252727&partnerID=8YFLogxK
U2 - 10.1007/s00467-002-0970-7
DO - 10.1007/s00467-002-0970-7
M3 - Article
C2 - 12478356
AN - SCOPUS:12244252727
SN - 0931-041X
VL - 17
SP - 1047
EP - 1052
JO - Pediatric Nephrology
JF - Pediatric Nephrology
IS - 12
ER -