Plasmid detection and sizing in single colony lysates

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116 Scopus citations

Abstract

A simple and contained procedure for the rapid assay of the presence and size of plasmids similar to Col E1 is described. Bacteria are picked from an agar plate with a toothpick, lysed with dodecyl sulfate and heat, and placed directly on an agarose gel for electrophoresis.

Original languageEnglish
Pages (from-to)393-394
Number of pages2
JournalScience
Volume195
Issue number4276
DOIs
StatePublished - 1977

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