TY - JOUR
T1 - Plasma angiopoietin-2 levels increase in children following cardiopulmonary bypass
AU - Giuliano, John S.
AU - Lahni, Patrick M.
AU - Bigham, Michael T.
AU - Manning, Peter B.
AU - Nelson, David P.
AU - Wong, Hector R.
AU - Wheeler, Derek S.
N1 - Funding Information:
Acknowledgments We would like thank Tracey VanVliet and Lois Bogenschutz in the Cardiology research department at Cincinnati Children’s Hospital for their assistance with this project. The study was supported by the National Institutes of Health KO8 GM077432 (DSW).
PY - 2008/10
Y1 - 2008/10
N2 - Objective: The aim was to investigate the effects of cardiopulmonary bypass (CPB) on plasma levels of the vascular growth factors, angiopoietin (angpt)-1, angpt-2, and vascular endothelial growth factor (VEGF). Design: The design was a prospective, clinical investigation. Setting: The setting was a 12-bed pediatric cardiac intensive care unit of a tertiary children's medical center. Patients: The patients were 48 children (median age, 5 months) undergoing surgical correction or palliation of congenital heart disease who were prospectively enrolled following informed consent. Interventions: There were no interventions in this study. Measurements and results: Plasma samples were obtained at baseline and at 0, 6, and 24 h following CPB. Angpt-1, angpt-2, and VEGF levels were measured via commercial ELISA. Angpt-2 levels increased by 6 h (0.95, IQR 0.43-2.08 ng mL-1 vs. 4.62, IQR 1.16-6.93 ng mL -1, P < 0.05) and remained significantly elevated at 24 h after CPB (1.85, IQR 0.70-2.76 ng mL-1; P < 0.05). Angpt-1 levels remained unchanged immediately after CPB, but were significantly decreased at 24 h after CPB (0.64, IQR 0.40-1.62 ng mL-1 vs. 1.99, IQR 1.23-2.63 ng mL-1, P < 0.05). Angpt-2 levels correlated significantly with cardiac intensive care unit (CICU) length of stay (LOS) and were an independent predictor for CICU LOS on subsequent multivariate analysis. Conclusions: Angpt-2 appears to be an important biomarker of adverse outcome following CPB in children.
AB - Objective: The aim was to investigate the effects of cardiopulmonary bypass (CPB) on plasma levels of the vascular growth factors, angiopoietin (angpt)-1, angpt-2, and vascular endothelial growth factor (VEGF). Design: The design was a prospective, clinical investigation. Setting: The setting was a 12-bed pediatric cardiac intensive care unit of a tertiary children's medical center. Patients: The patients were 48 children (median age, 5 months) undergoing surgical correction or palliation of congenital heart disease who were prospectively enrolled following informed consent. Interventions: There were no interventions in this study. Measurements and results: Plasma samples were obtained at baseline and at 0, 6, and 24 h following CPB. Angpt-1, angpt-2, and VEGF levels were measured via commercial ELISA. Angpt-2 levels increased by 6 h (0.95, IQR 0.43-2.08 ng mL-1 vs. 4.62, IQR 1.16-6.93 ng mL -1, P < 0.05) and remained significantly elevated at 24 h after CPB (1.85, IQR 0.70-2.76 ng mL-1; P < 0.05). Angpt-1 levels remained unchanged immediately after CPB, but were significantly decreased at 24 h after CPB (0.64, IQR 0.40-1.62 ng mL-1 vs. 1.99, IQR 1.23-2.63 ng mL-1, P < 0.05). Angpt-2 levels correlated significantly with cardiac intensive care unit (CICU) length of stay (LOS) and were an independent predictor for CICU LOS on subsequent multivariate analysis. Conclusions: Angpt-2 appears to be an important biomarker of adverse outcome following CPB in children.
KW - Cardiopulmonary bypass
KW - Congenital heart disease
KW - Inflammation
KW - Pediatrics
KW - Systemic inflammatory response syndrome (SIRS)
KW - Vascular growth factor
UR - http://www.scopus.com/inward/record.url?scp=52949126714&partnerID=8YFLogxK
U2 - 10.1007/s00134-008-1174-9
DO - 10.1007/s00134-008-1174-9
M3 - Article
C2 - 18516587
AN - SCOPUS:52949126714
SN - 0342-4642
VL - 34
SP - 1851
EP - 1857
JO - Intensive care medicine
JF - Intensive care medicine
IS - 10
ER -