Planning and analysis of axon degeneration screening experiments

Lyndah Lovell, John Bramley, William Buchser

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review


A network of intersecting molecular pathways interacts to initiate and execute axon destruction. Maximum protection against axon degeneration likely requires more than manipulation of a single target. Here, we describe the process of designing a high-throughput arrayed screening assay for the identification of key factors responsible for axon destruction and/or protection. First, we go over some existing screens in the literature, then discuss the planning, tracking, analysis, and statistics around such a screening experiment. Prioritization of perturbations may allow laboratories to cost-effectively explore the process of screening. We also present the pairing of a combinatorial drug screen with a machine learning algorithm, predicting how to best modulate neurodegenerative and neuroprotective components.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages20
StatePublished - 2020

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Analysis
  • Antitargets
  • Axon degeneration
  • Discovery
  • High-throughput
  • Hits
  • Nonhits
  • Screening
  • Support vector machine learning
  • Targets


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