@inbook{986fb0c9269243cda62c80619c7240f7,
title = "Planning and analysis of axon degeneration screening experiments",
abstract = "A network of intersecting molecular pathways interacts to initiate and execute axon destruction. Maximum protection against axon degeneration likely requires more than manipulation of a single target. Here, we describe the process of designing a high-throughput arrayed screening assay for the identification of key factors responsible for axon destruction and/or protection. First, we go over some existing screens in the literature, then discuss the planning, tracking, analysis, and statistics around such a screening experiment. Prioritization of perturbations may allow laboratories to cost-effectively explore the process of screening. We also present the pairing of a combinatorial drug screen with a machine learning algorithm, predicting how to best modulate neurodegenerative and neuroprotective components.",
keywords = "Analysis, Antitargets, Axon degeneration, Discovery, High-throughput, Hits, Nonhits, Screening, Support vector machine learning, Targets",
author = "Lyndah Lovell and John Bramley and William Buchser",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2020.",
year = "2020",
doi = "10.1007/978-1-0716-0585-1_6",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "63--82",
booktitle = "Methods in Molecular Biology",
}