TY - JOUR
T1 - Physiological and biochemical effects of 17β estradiol in aging female rat brain
AU - Kumar, Pardeep
AU - Taha, Asia
AU - Kale, R. K.
AU - Cowsik, S. M.
AU - Baquer, Najma Zaheer
N1 - Funding Information:
The author Pardeep Kumar is grateful to the financial support from Council of Scientific and Industrial Research in the form of Senior Research Fellowship. Financial grant from Indian Council of Medical Research and University Grant Commission , New Delhi, India in the form of projects are acknowledged. GLUT4 antibody was a kind gift from Dr. Sameul Cushman (National Institute of Health, USA). Special thanks to late Mr. Amit Kumar Sharma, for his valuable suggestions. The authors wish to express their appreciation to Prof. P.McLean, University College London Medical School, London for her invaluable suggestions; Dr. Krishna Prakash and Dr. Somnath Mukherjee for their help in Molecular Biology experiments. Prof. Najma Z. Baquer is also a visiting Professor at Jamia Milia Islamia University, New Delhi.
PY - 2011/7
Y1 - 2011/7
N2 - Aging in females and males is considered as the end of natural protection against age related diseases like osteoporosis, coronary heart disease, diabetes, Alzheimer's disease and Parkinson's disease. These changes increase during menopausal condition in females when the level of estradiol is decreased. The objective of this study was to observe the changes in activities of monoamine oxidase, glucose transporter-4 levels, membrane fluidity, lipid peroxidation levels and lipofuscin accumulation occurring in brains of female rats of 3. months (young), 12. months (adult) and 24. months (old) age groups, and to see whether these changes are restored to normal levels after exogenous administration of estradiol (0.1 μg/g body weight for 1. month). The results obtained in the present work revealed that normal aging was associated with significant increases in the activity of monoamine oxidase, lipid peroxidation levels and lipofuscin accumulation in the brains of aging female rats, and a decrease in glucose transporter-4 level and membrane fluidity. Our data showed that estradiol treatment significantly decreased monoamine oxidase activity, lipid peroxidation and lipofuscin accumulation in brain regions of aging rats, and a reversal of glucose transporter-4 levels and membrane fluidity was achieved, therefore it can be concluded from the present findings that estradiol's beneficial effects seemed to arise from its antilipofuscin, antioxidant and antilipidperoxidative effects, implying an overall anti-aging action. The results of this study will be useful for pharmacological modification of the aging process and applying new strategies for control of age related disorders.
AB - Aging in females and males is considered as the end of natural protection against age related diseases like osteoporosis, coronary heart disease, diabetes, Alzheimer's disease and Parkinson's disease. These changes increase during menopausal condition in females when the level of estradiol is decreased. The objective of this study was to observe the changes in activities of monoamine oxidase, glucose transporter-4 levels, membrane fluidity, lipid peroxidation levels and lipofuscin accumulation occurring in brains of female rats of 3. months (young), 12. months (adult) and 24. months (old) age groups, and to see whether these changes are restored to normal levels after exogenous administration of estradiol (0.1 μg/g body weight for 1. month). The results obtained in the present work revealed that normal aging was associated with significant increases in the activity of monoamine oxidase, lipid peroxidation levels and lipofuscin accumulation in the brains of aging female rats, and a decrease in glucose transporter-4 level and membrane fluidity. Our data showed that estradiol treatment significantly decreased monoamine oxidase activity, lipid peroxidation and lipofuscin accumulation in brain regions of aging rats, and a reversal of glucose transporter-4 levels and membrane fluidity was achieved, therefore it can be concluded from the present findings that estradiol's beneficial effects seemed to arise from its antilipofuscin, antioxidant and antilipidperoxidative effects, implying an overall anti-aging action. The results of this study will be useful for pharmacological modification of the aging process and applying new strategies for control of age related disorders.
KW - Brain aging
KW - Endocrine
KW - Estrogen
KW - Glucose transporter
KW - Membrane fluidity
KW - Neurodegenerative diseases
UR - https://www.scopus.com/pages/publications/79956302996
U2 - 10.1016/j.exger.2011.02.008
DO - 10.1016/j.exger.2011.02.008
M3 - Article
C2 - 21377519
AN - SCOPUS:79956302996
SN - 0531-5565
VL - 46
SP - 597
EP - 605
JO - Experimental Gerontology
JF - Experimental Gerontology
IS - 7
ER -