Physical mapping of loci in the distal half of the short arm of the human X chromosome: Implications for the spreading of X-chromosome inactivation

J. C. Wang, M. B. Passage, J. Ellison, M. A. Becker, P. H. Yen, L. J. Shapiro, T. K. Mohandas

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    9 Scopus citations

    Abstract

    The relative order of 11 loci in the distal half of the short arm of the human X chromosome was examined using a panel of somatic cell hybrids containing structurally rearranged X chromosomes. The results show that the gene for phosphoribosylpyrophosphate synthetase 2 (PRPS2) is located between ZFX (zinc finger protein, X-linked) and STS (steroid sulfatase). The results also confirm the localization of ZFX distal to POLA (α-DNA polymerase). Previous studies have shown that STS and ZFX escape X-inactivation whereas POLA undergoes inactivation. Evaluation of PRPS2 expression in somatic cell hybrids containing inactive human X chromosomes showed that PRPS2 undergoes X-inactivation. These results provide further evidence for interspersion of loci that do and do not undergo X-inactivation on the human X chromosome.

    Original languageEnglish
    Pages (from-to)195-200
    Number of pages6
    JournalSomatic Cell and Molecular Genetics
    Volume18
    Issue number2
    DOIs
    StatePublished - Mar 1992

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