TY - JOUR
T1 - Physical and transcript map of the hereditary prostate cancer region at Xq27
AU - Stephan, Dietrich A.
AU - Howell, Gareth R.
AU - Teslovich, Tanya M.
AU - Coffey, Alison J.
AU - Smith, Lorie
AU - Bailey-Wilson, Joan E.
AU - Malechek, Lindsay
AU - Gildea, Derek
AU - Smith, Jeffrey R.
AU - Gillanders, Elizabeth M.
AU - Schleutker, Johanna
AU - Hu, Ping
AU - Steingruber, Helen E.
AU - Dhami, Pawandeep
AU - Robbins, Christiane M.
AU - Makalowska, Izabela
AU - Carpten, John D.
AU - Sood, Raman
AU - Mumm, Steve
AU - Reinbold, Rolland
AU - Bonner, Tom I.
AU - Baffoe-Bonnie, Agnes
AU - Bubendorf, Lukas
AU - Heiskanen, Mervi
AU - Kallioneimi, Olli P.
AU - Baxevanis, Andreas D.
AU - Joseph, Shirin S.
AU - Zucchi, Ileana
AU - Burk, Robert D.
AU - Isaacs, William
AU - Ross, Mark T.
AU - Trent, Jeffrey M.
PY - 2002/1
Y1 - 2002/1
N2 - We have recently mapped a locus for hereditary prostate cancer (termed HPCX) to the long arm of the X chromosome (Xq25-q27) through a genome-wide linkage study. Here we report the construction of an ∼9-Mb sequence-ready bacterial clone contig map of Xq26.3-q27.3. The contig was constructed by screening BAC/PAC libraries with markers spaced at ∼85-kb intervals. We identified overlapping clones by end-sequencing framework clones to generate 407 new sequence-tagged sites, followed by PCR verification of overlaps. Contig assembly was based on clone restriction fingerprinting and the landmark information. We identified a minimal overlap contig for genomic sequencing, which has yielded 7.7 Mb of finished sequence and 1.5 Mb of draft sequence. The transcriptional mapping effort localized 57 known and predicted genes by database searching, STS content mapping, and sequencing, followed by sequence annotation. These transcriptional units represent candidate genes for HPCX and multiple other hereditary diseases at Xq26.3-q27.3.
AB - We have recently mapped a locus for hereditary prostate cancer (termed HPCX) to the long arm of the X chromosome (Xq25-q27) through a genome-wide linkage study. Here we report the construction of an ∼9-Mb sequence-ready bacterial clone contig map of Xq26.3-q27.3. The contig was constructed by screening BAC/PAC libraries with markers spaced at ∼85-kb intervals. We identified overlapping clones by end-sequencing framework clones to generate 407 new sequence-tagged sites, followed by PCR verification of overlaps. Contig assembly was based on clone restriction fingerprinting and the landmark information. We identified a minimal overlap contig for genomic sequencing, which has yielded 7.7 Mb of finished sequence and 1.5 Mb of draft sequence. The transcriptional mapping effort localized 57 known and predicted genes by database searching, STS content mapping, and sequencing, followed by sequence annotation. These transcriptional units represent candidate genes for HPCX and multiple other hereditary diseases at Xq26.3-q27.3.
KW - Bacterial artificial chromosomes
KW - Contig mapping
KW - Genome mapping
KW - Genome sequencing
KW - P1 artificial chromosomes
KW - Physical mapping
KW - Prostate cancer
KW - Repetitive elements
KW - Sequence annotation
KW - Xq26.3-q27.3
UR - https://www.scopus.com/pages/publications/0035701501
U2 - 10.1006/geno.2001.6681
DO - 10.1006/geno.2001.6681
M3 - Article
C2 - 11827456
AN - SCOPUS:0035701501
SN - 0888-7543
VL - 79
SP - 41
EP - 50
JO - Genomics
JF - Genomics
IS - 1
ER -