TY - JOUR
T1 - Photoconversion in orange and red fluorescent proteins
AU - Kremers, Gert Jan
AU - Hazelwood, Kristin L.
AU - Murphy, Christopher S.
AU - Davidson, Michael W.
AU - Piston, David W.
N1 - Funding Information:
We thank D.M. Chudakov (Shemiakin-Ovchinnikov Institute of Bioorganic Chemistry,), A. Miyawaki (RIKEN Brain Science Institute) and R.Y. Tsien (University of California at San Diego) for providing plasmids encoding fluorescent protein variants. Measurements were performed in part at the Vanderbilt University Medical Center Cell Imaging Shared Resource (National Institutes of Health grants CA68485, DK20593, DK58404) and at the Quantitative Fluorescence Microcopy course at Mount Desert Island Biological Lab. We thank G. Daniels (Leica), J. Wailes (Zeiss), B. Burklow (Olympus) and S. Schwartz (Nikon) for assistance with their microscopes, and B. Livesay for help with the mVenus experiments. This work was supported by National Institutes of Health grant GM72048 (to D.W.P.).
PY - 2009
Y1 - 2009
N2 - We found that photoconversion is fairly common among orange and red fluorescent proteins, as in a screen of 12 proteins, 8 exhibited photoconversion. Specifically, three red fluorescent proteins could be switched to a green state, and two orange variants could be photoconverted to a far-red state. The orange proteins are ideal for dual-probe highlighter applications, and they exhibited the most red-shifted excitation of all fluorescent proteins described to date.
AB - We found that photoconversion is fairly common among orange and red fluorescent proteins, as in a screen of 12 proteins, 8 exhibited photoconversion. Specifically, three red fluorescent proteins could be switched to a green state, and two orange variants could be photoconverted to a far-red state. The orange proteins are ideal for dual-probe highlighter applications, and they exhibited the most red-shifted excitation of all fluorescent proteins described to date.
UR - http://www.scopus.com/inward/record.url?scp=67349229144&partnerID=8YFLogxK
U2 - 10.1038/nmeth.1319
DO - 10.1038/nmeth.1319
M3 - Article
C2 - 19363494
AN - SCOPUS:67349229144
SN - 1548-7091
VL - 6
SP - 355
EP - 358
JO - Nature Methods
JF - Nature Methods
IS - 5
ER -