Photoconversion in orange and red fluorescent proteins

Gert Jan Kremers, Kristin L. Hazelwood, Christopher S. Murphy, Michael W. Davidson, David W. Piston

Research output: Contribution to journalArticle

93 Scopus citations

Abstract

We found that photoconversion is fairly common among orange and red fluorescent proteins, as in a screen of 12 proteins, 8 exhibited photoconversion. Specifically, three red fluorescent proteins could be switched to a green state, and two orange variants could be photoconverted to a far-red state. The orange proteins are ideal for dual-probe highlighter applications, and they exhibited the most red-shifted excitation of all fluorescent proteins described to date.

Original languageEnglish
Pages (from-to)355-358
Number of pages4
JournalNature Methods
Volume6
Issue number5
DOIs
StatePublished - Apr 13 2009
Externally publishedYes

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    Kremers, G. J., Hazelwood, K. L., Murphy, C. S., Davidson, M. W., & Piston, D. W. (2009). Photoconversion in orange and red fluorescent proteins. Nature Methods, 6(5), 355-358. https://doi.org/10.1038/nmeth.1319