Photocontrol of coiled-coil proteins in living cells

Fuzhong Zhang; Katharina A. Timm; Katja M. Arndt; G. Andrew Woolley

doi:10.1002/anie.201000909

Photocontrol of coiled-coil proteins in living cells

Fuzhong Zhang, Katharina A. Timm, Katja M. Arndt, G. Andrew Woolley

Research output: Contribution to journal › Article › peer-review

100 Scopus citations

Abstract

Light switching of the activity of a coiledcoil protein, the AP-1 transcription factor, in living cells was made possible by the introduction of a designed azobenzenecross-linked dominant negative peptide, XAFosW (red and yellow in the picture). In the dark, XAFosW showed decreased helical content and decreased affinity for target Jun proteins (green); irradiation at 365 nm enhanced helicity and target affinity. Chemical Equation Presentation

Original language	English
Pages (from-to)	3943-3946
Number of pages	4
Journal	Angewandte Chemie - International Edition
Volume	49
Issue number	23
DOIs	https://doi.org/10.1002/anie.201000909
State	Published - May 25 2010

Keywords

Azobenzene
Isomerization
Leucine zipper
Peptides
Photoswitches

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10.1002/anie.201000909

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abstract = "Light switching of the activity of a coiledcoil protein, the AP-1 transcription factor, in living cells was made possible by the introduction of a designed azobenzenecross-linked dominant negative peptide, XAFosW (red and yellow in the picture). In the dark, XAFosW showed decreased helical content and decreased affinity for target Jun proteins (green); irradiation at 365 nm enhanced helicity and target affinity. Chemical Equation Presentation",

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author = "Fuzhong Zhang and Timm, {Katharina A.} and Arndt, {Katja M.} and Woolley, {G. Andrew}",

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AU - Zhang, Fuzhong

AU - Timm, Katharina A.

AU - Arndt, Katja M.

AU - Woolley, G. Andrew

PY - 2010/5/25

Y1 - 2010/5/25

N2 - Light switching of the activity of a coiledcoil protein, the AP-1 transcription factor, in living cells was made possible by the introduction of a designed azobenzenecross-linked dominant negative peptide, XAFosW (red and yellow in the picture). In the dark, XAFosW showed decreased helical content and decreased affinity for target Jun proteins (green); irradiation at 365 nm enhanced helicity and target affinity. Chemical Equation Presentation

AB - Light switching of the activity of a coiledcoil protein, the AP-1 transcription factor, in living cells was made possible by the introduction of a designed azobenzenecross-linked dominant negative peptide, XAFosW (red and yellow in the picture). In the dark, XAFosW showed decreased helical content and decreased affinity for target Jun proteins (green); irradiation at 365 nm enhanced helicity and target affinity. Chemical Equation Presentation

KW - Azobenzene

KW - Isomerization

KW - Leucine zipper

KW - Peptides

KW - Photoswitches

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U2 - 10.1002/anie.201000909

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JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

IS - 23

ER -

Photocontrol of coiled-coil proteins in living cells

Abstract

Keywords

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