Photobleaching-free infrared near-field microscopy localizes molecules in neurons

J. Generosi, G. Margaritondo, M. Kropf, H. Hirling, S. Catsicas, K. Johnsson, N. H. Tolk, D. W. Piston, A. Cricenti

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7 Scopus citations


High-resolution detection of specific molecules in cells is a major challenge in biology. We show that infrared scanning near-field microscopy can detect the spatial distribution of α -amino-3-hydroxy-5-methyl-4- isoxazole-propionic acid-type glutamate receptor clusters on hippocampal neurons. The GluR2 subunits were labeled with the die Alexa 488 and high-resolution infrared micrographs were taken at 6.25 μm. The absence of photobleaching makes this approach suitable for a long-term observation and allows to localize different infrared-absorbing molecules over the complex background of other cell components.

Original languageEnglish
Article number106102
JournalJournal of Applied Physics
Issue number10
StatePublished - 2008


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