TY - GEN
T1 - Photoacoustic microscopy using Evans Blue dye as a contrast agent
AU - Yao, Junjie
AU - Maslov, Konstantin I.
AU - Hu, Song
AU - Wang, Lihong V.
PY - 2010/2/23
Y1 - 2010/2/23
N2 - Complete and continuous imaging of microvascular networks is crucial for a wide variety of biomedical applications. Photoacoustic tomography can provide high resolution microvascular imaging using hemoglobin within red blood cells (RBC) as an endogenous contrast agent. However, intermittent RBC flow in capillaries results in discontinuous and fragmentary capillary images. To overcome this problem, we used Evans Blue (EB) dye as a contrast agent for in vivo photoacoustic imaging. EB has strong optical absorption at 610 nm and distributes uniformly in the blood stream by chemically binding to albumin. By intravenous injection of EB (6%, 200 μL), complete and continuous microvascular networks-especially capillaries-of the ears of nude mice were imaged. The diffusion of EB (3%, 100 μL) leaving the blood stream was monitored for 2 hours. At lower administration dose of EB (3%, 50 μL), the clearance of the EB-albumin complex was imaged for 10 days and quantitatively investigated using a two-compartment model.
AB - Complete and continuous imaging of microvascular networks is crucial for a wide variety of biomedical applications. Photoacoustic tomography can provide high resolution microvascular imaging using hemoglobin within red blood cells (RBC) as an endogenous contrast agent. However, intermittent RBC flow in capillaries results in discontinuous and fragmentary capillary images. To overcome this problem, we used Evans Blue (EB) dye as a contrast agent for in vivo photoacoustic imaging. EB has strong optical absorption at 610 nm and distributes uniformly in the blood stream by chemically binding to albumin. By intravenous injection of EB (6%, 200 μL), complete and continuous microvascular networks-especially capillaries-of the ears of nude mice were imaged. The diffusion of EB (3%, 100 μL) leaving the blood stream was monitored for 2 hours. At lower administration dose of EB (3%, 50 μL), the clearance of the EB-albumin complex was imaged for 10 days and quantitatively investigated using a two-compartment model.
KW - Diffusion dynamics
KW - Evans Blue dye
KW - Evans Blue-Albumin complex
KW - Optical-resolution photoacoustic microscopy
UR - https://www.scopus.com/pages/publications/77951537431
U2 - 10.1117/12.840684
DO - 10.1117/12.840684
M3 - Conference contribution
AN - SCOPUS:77951537431
SN - 9780819479600
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Photons Plus Ultrasound
PB - SPIE
T2 - Photons Plus Ultrasound: Imaging and Sensing 2010
Y2 - 24 January 2010 through 26 January 2010
ER -