Abstract
Quantitative assessment of the influence of general anesthetics on cerebral hemodynamics and oxygen metabolism at the microscopic level has been a long-standing challenge in brain research. The newly developed head-restrained photoacoustic microscopy (PAM) enables, for the first time, simultaneous label-free imaging of blood perfusion, oxygenation, and flow in single microvessels of the awake mouse brain. Furthermore, vessel segmentation analysis of the multiparametric PAM images enables the quantification of regional cerebral oxygen extraction fraction and metabolism. This enabling technology allows comprehensive characterization of the cerebral hemodynamic and metabolic responses to general anesthetics and holds great potential for studying neurovascular coupling in the awake brain and anesthetic neuroprotection against cerebral hypoxia and ischemia.
| Original language | English |
|---|---|
| Title of host publication | Neurophotonics and Biomedical Spectroscopy |
| Publisher | Elsevier |
| Pages | 215-227 |
| Number of pages | 13 |
| ISBN (Electronic) | 9780323480673 |
| ISBN (Print) | 9780323480680 |
| DOIs | |
| State | Published - Jan 1 2018 |
Keywords
- Awake brain imaging
- Cerebral hemodynamics
- General anesthetics
- Oxygen metabolism
- Photoacoustic microscopy