@article{9ea7fc4079f34437a07ab88d13de1ae6,
title = "Photo-activatable Cre recombinase regulates gene expression in vivo",
abstract = "Techniques allowing precise spatial and temporal control of gene expression in the brain are needed. Herein we describe optogenetic approaches using a photo-activatable Cre recombinase (PA-Cre) to stably modify gene expression in the mouse brain. Blue light illumination for 12hours via optical fibers activated PA-Cre in the hippocampus, a deep brain structure. Two-photon illumination through a thinned skull window for 100minutes activated PA-Cre within a sub-millimeter region of cortex. Light activation of PA-Cre may allow permanent gene modification with improved spatiotemporal precision compared to standard methods.",
author = "Schindler, \{Suzanne E.\} and McCall, \{Jordan G.\} and Ping Yan and Hyrc, \{Krzystof L.\} and Mingjie Li and Tucker, \{Chandra L.\} and Lee, \{Jin Moo\} and Bruchas, \{Michael R.\} and Diamond, \{Marc I.\}",
note = "Funding Information: S.E.S. was supported by a Clinical Research Training Fellowship from the American Brain Foundation and the Alzheimer{\textquoteright}s Association and a NIH K career development award (5K12 HD001459-15). J.G.M. was supported by a Ruth L. Kirschstein National Research Service Award (F31 MH101956). Research funding was provided by the McDonnell Center for Systems Neuroscience at Washington University in St. Louis (M.I.D) and (M.R.B). This work was supported by the Hope Center Alafi Neuroimaging Lab, a P30 Neuroscience Blueprint Interdisciplinary Center Core award to Washington University (P30 NS057105), and a NIH EUREKA grant (R01DA037152, to M.R.B). The authors thank Larisa Belaygorod for technical expertise in culturing primary neurons, Sherry Clark and Lamley Lawson for mouse husbandry, Brandon Holmes for flow cytometry expertise, Sarah Kaufman for helpful suggestions, Aimin Li for cloning expertise and Anne Fagan for critical review.",
year = "2015",
month = sep,
day = "9",
doi = "10.1038/srep13627",
language = "English",
volume = "5",
journal = "Scientific reports",
issn = "2045-2322",
}