Phosphorylation of TIP60 suppresses 53BP1 localization at DNA damage sites

Mischa Longyin Li, Qinqin Jiang, Natarajan V. Bhanu, Junmin Wu, Weihua Li, Benjamin A. Garcia, Roger A. Greenberg

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

A proper balance between the repair of DNA double-strand breaks (DSBs) by homologous recombination and nonhomologous end joining is critical for maintaining genome integrity and preventing tumorigenesis. This balance is regulated and fine-tuned by a variety of factors, including cell cycle and the chromatin environment. The histone acetyltransferase TIP60 was previously shown to suppress pathological end joining and promote homologous recombination. However, it is unknown how regulatory posttranslational modifications impact TIP60 acetyltransferase activity to influence the outcome of DSB responses. In this study, we report that phosphorylation of TIP60 on serines 90 and 86 is important for limiting the accumulation of the pro-end joining factor 53BP1 at DSBs in S and G 2 cell cycle phases. Mutation of these sites disrupts histone acetylation changes in response to DNA damage, BRCA1 localization to DSBs, and poly(ADP-ribose) polymerase (PARP) inhibitor resistance. These findings reveal that phosphorylation directs TIP60-dependent acetylation to promote homologous recombination and maintain genome stability.

Original languageEnglish
Article numbere00209-18
JournalMolecular and cellular biology
Volume39
Issue number1
DOIs
StatePublished - Jan 1 2019

Keywords

  • 53BP1
  • Acetylation
  • BRCA1
  • DNA repair
  • Homologous recombination
  • TIP60

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