TY - JOUR
T1 - Phosphorylation of the IgE receptor from ionophore A23187 stimulated intact rat mast cells
AU - Hempstead, Barbara L.
AU - Kulczycki, Anthony
AU - Parker, Charles W.
N1 - Funding Information:
Acknowledgements: We would like to thank Dr. T.J. Sullivan for performing the histamine assays and Dr. A. McGee for invaluable advice and assistance with paper chromatography. This work was supported in part by grants 1 R01 AI 16946, 5 T32 GM 7200 and P50 AI 15322 from the National Institutes of Health.
PY - 1981/2/12
Y1 - 1981/2/12
N2 - Purified rat serosal mast cells were labeled either with [32P]orthophosphate or [35S]methionine and their receptors for immunoglobulin E were isolated by repetitive affinity chromatography. In 35S-labeled receptor preparations SDS polyacrylamide gels revealed a broad receptor band, Mr 45,000 to 53,000, and two other bands, Mr 30,000 and 16,000, which apparently represent receptor-associated proteins. Only the receptor band was labeled by 32P. Phosphorylation of receptor was markedly stimulated by the divalent cation ionophore A23187, a known stimulator of histamine release, with changes occuring as early as 15 seconds. This early increase in receptor phosphorylation may be involved in the control of mediator secretion.
AB - Purified rat serosal mast cells were labeled either with [32P]orthophosphate or [35S]methionine and their receptors for immunoglobulin E were isolated by repetitive affinity chromatography. In 35S-labeled receptor preparations SDS polyacrylamide gels revealed a broad receptor band, Mr 45,000 to 53,000, and two other bands, Mr 30,000 and 16,000, which apparently represent receptor-associated proteins. Only the receptor band was labeled by 32P. Phosphorylation of receptor was markedly stimulated by the divalent cation ionophore A23187, a known stimulator of histamine release, with changes occuring as early as 15 seconds. This early increase in receptor phosphorylation may be involved in the control of mediator secretion.
UR - http://www.scopus.com/inward/record.url?scp=0019482602&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(81)91184-0
DO - 10.1016/0006-291X(81)91184-0
M3 - Article
C2 - 6452878
AN - SCOPUS:0019482602
SN - 0006-291X
VL - 98
SP - 815
EP - 822
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -