Phosphorylation of the IgE receptor from ionophore A23187 stimulated intact rat mast cells

Barbara L. Hempstead, Anthony Kulczycki, Charles W. Parker

Research output: Contribution to journalArticle

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Abstract

Purified rat serosal mast cells were labeled either with [32P]orthophosphate or [35S]methionine and their receptors for immunoglobulin E were isolated by repetitive affinity chromatography. In 35S-labeled receptor preparations SDS polyacrylamide gels revealed a broad receptor band, Mr 45,000 to 53,000, and two other bands, Mr 30,000 and 16,000, which apparently represent receptor-associated proteins. Only the receptor band was labeled by 32P. Phosphorylation of receptor was markedly stimulated by the divalent cation ionophore A23187, a known stimulator of histamine release, with changes occuring as early as 15 seconds. This early increase in receptor phosphorylation may be involved in the control of mediator secretion.

Original languageEnglish
Pages (from-to)815-822
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume98
Issue number3
DOIs
StatePublished - Feb 12 1981

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