TY - JOUR
T1 - Phosphorus acquisition efficiency in arbuscular mycorrhizal maize is correlated with the abundance of root-external hyphae and the accumulation of transcripts encoding PHT1 phosphate transporters
AU - Sawers, Ruairidh J.H.
AU - Svane, Simon F.
AU - Quan, Clement
AU - Grønlund, Mette
AU - Wozniak, Barbara
AU - Gebreselassie, Mesfin Nigussie
AU - González-Muñoz, Eliécer
AU - Chávez Montes, Ricardo A.
AU - Baxter, Ivan
AU - Goudet, Jerome
AU - Jakobsen, Iver
AU - Paszkowski, Uta
N1 - Publisher Copyright:
© 2017 The Authors. New Phytologist © 2017 New Phytologist Trust
PY - 2017/4/1
Y1 - 2017/4/1
N2 - Plant interactions with arbuscular mycorrhizal fungi have long attracted interest for their potential to promote more efficient use of mineral resources in agriculture. Their use, however, remains limited by a lack of understanding of the processes that determine the outcome of the symbiosis. In this study, the impact of host genotype on growth response to mycorrhizal inoculation was investigated in a panel of diverse maize lines. A panel of 30 maize lines was evaluated with and without inoculation with arbuscular mycorrhizal fungi. The line Oh43 was identified to show superior response and, along with five other reference lines, was characterized in greater detail in a split-compartment system, using 33P to quantify mycorrhizal phosphorus uptake. Changes in relative growth indicated variation in host capacity to profit from the symbiosis. Shoot phosphate content, abundance of root-internal and -external fungal structures, mycorrhizal phosphorus uptake, and accumulation of transcripts encoding plant PHT1 family phosphate transporters varied among lines. Superior response in Oh43 is correlated with extensive development of root-external hyphae, accumulation of specific Pht1 transcripts and high phosphorus uptake by mycorrhizal plants. The data indicate that host genetic factors influence fungal growth strategy with an impact on plant performance.
AB - Plant interactions with arbuscular mycorrhizal fungi have long attracted interest for their potential to promote more efficient use of mineral resources in agriculture. Their use, however, remains limited by a lack of understanding of the processes that determine the outcome of the symbiosis. In this study, the impact of host genotype on growth response to mycorrhizal inoculation was investigated in a panel of diverse maize lines. A panel of 30 maize lines was evaluated with and without inoculation with arbuscular mycorrhizal fungi. The line Oh43 was identified to show superior response and, along with five other reference lines, was characterized in greater detail in a split-compartment system, using 33P to quantify mycorrhizal phosphorus uptake. Changes in relative growth indicated variation in host capacity to profit from the symbiosis. Shoot phosphate content, abundance of root-internal and -external fungal structures, mycorrhizal phosphorus uptake, and accumulation of transcripts encoding plant PHT1 family phosphate transporters varied among lines. Superior response in Oh43 is correlated with extensive development of root-external hyphae, accumulation of specific Pht1 transcripts and high phosphorus uptake by mycorrhizal plants. The data indicate that host genetic factors influence fungal growth strategy with an impact on plant performance.
KW - PHT1
KW - arbuscular mycorrhiza (AM)
KW - maize
KW - phosphorus
KW - root-external hyphae
UR - http://www.scopus.com/inward/record.url?scp=85015809451&partnerID=8YFLogxK
U2 - 10.1111/nph.14403
DO - 10.1111/nph.14403
M3 - Article
C2 - 28098948
AN - SCOPUS:85015809451
SN - 0028-646X
VL - 214
SP - 632
EP - 643
JO - New Phytologist
JF - New Phytologist
IS - 2
ER -