TY - JOUR
T1 - Phosphorus-31 NMR of rat brain in vivo with bloodless perfluorocarbon perfused rat
AU - Ackerman, Joseph J.H.
AU - Berkowitz, Bruce A.
AU - Deuel, Ruthmary K.
N1 - Funding Information:
Extremely helpful, detailed regarding the art of perfluorocdrbon fully acknowledged. Support for this work was provided by Washinyton Funds, and NIH yrdnts GM30331, RR07054, and KR0538Y. use of Oracle software provided by Professor Georye University; NIH Biotechnolgy Research Resource, grant
PY - 1984/3/30
Y1 - 1984/3/30
N2 - Rat brain in vivo has been examined by 31p NMR under conditions of normal blood perfusion (hematocrit 38%) and under conditions in which a perfluorocarbon blood substitute, devoid of any phosphorus containing compounds, largely replaced the animal's normal blood supply (hematocrit 7%). These studies demonstate that 2,3-diphosphoglycerate does not - as has been suggested - contribute to, and thus does not interfere with, the 31p NMR analysis of rat brain in vivo. However, low intensity 31P resonances assigned to choline phosphate, glycerol 3-phosphorylethanolamine, and glycerol 3-phosphorylcholine are observed. "High energy phosphorus" metabolite levels show no marked change over two hours with perfluorocarbon blood substitution from those of the normal blood perfused animal. This supports use of perfluorocarbon media for tissue perfusion in vitro and for 19F NMR vascular imaging in vivo.
AB - Rat brain in vivo has been examined by 31p NMR under conditions of normal blood perfusion (hematocrit 38%) and under conditions in which a perfluorocarbon blood substitute, devoid of any phosphorus containing compounds, largely replaced the animal's normal blood supply (hematocrit 7%). These studies demonstate that 2,3-diphosphoglycerate does not - as has been suggested - contribute to, and thus does not interfere with, the 31p NMR analysis of rat brain in vivo. However, low intensity 31P resonances assigned to choline phosphate, glycerol 3-phosphorylethanolamine, and glycerol 3-phosphorylcholine are observed. "High energy phosphorus" metabolite levels show no marked change over two hours with perfluorocarbon blood substitution from those of the normal blood perfused animal. This supports use of perfluorocarbon media for tissue perfusion in vitro and for 19F NMR vascular imaging in vivo.
UR - http://www.scopus.com/inward/record.url?scp=0021259898&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(84)90860-X
DO - 10.1016/0006-291X(84)90860-X
M3 - Article
C2 - 6712676
AN - SCOPUS:0021259898
SN - 0006-291X
VL - 119
SP - 913
EP - 919
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -