Phosphorus-31 NMR of rat brain in vivo with bloodless perfluorocarbon perfused rat

Joseph J.H. Ackerman, Bruce A. Berkowitz, Ruthmary K. Deuel

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Rat brain in vivo has been examined by 31p NMR under conditions of normal blood perfusion (hematocrit 38%) and under conditions in which a perfluorocarbon blood substitute, devoid of any phosphorus containing compounds, largely replaced the animal's normal blood supply (hematocrit 7%). These studies demonstate that 2,3-diphosphoglycerate does not - as has been suggested - contribute to, and thus does not interfere with, the 31p NMR analysis of rat brain in vivo. However, low intensity 31P resonances assigned to choline phosphate, glycerol 3-phosphorylethanolamine, and glycerol 3-phosphorylcholine are observed. "High energy phosphorus" metabolite levels show no marked change over two hours with perfluorocarbon blood substitution from those of the normal blood perfused animal. This supports use of perfluorocarbon media for tissue perfusion in vitro and for 19F NMR vascular imaging in vivo.

Original languageEnglish
Pages (from-to)913-919
Number of pages7
JournalBiochemical and Biophysical Research Communications
Issue number3
StatePublished - Mar 30 1984


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