@article{e301cac60f7542acbb04df0f8839cffd,
title = "Phospholipid fatty acid composition of pulmonary airway epithelial cells: potential substrates for oxygenation",
abstract = "To determine possible substrates for airway epithelial lipoxygenase and cyclooxy genase activities, we examined the amounts and distributions of fatty acids in phospholipids of human, dog and sheep airway epithelial cells. We found that the cells contained significant levels of n - 6 and n - 3 fatty acids in species-specific amounts: dog cells were relatively enriched in the n - 6 series and poor in n - 3, while sheep cells were enriched in the n - 3 series and poor in n - 6. Despite differences in fatty acid content, cells from each species expressed a constant phospholipid composition and distributed their n - 6 and n - 3 fatty acids in a stereotyped fashion among phospholipid classes. The analysis shows that the species differences in oxygenation activities reported previously are paralleled by heterogeneity in potential substrates.",
keywords = "(Lung epithelial cell), Arachidonic acid, Cyclooxygenase, Linoleic acid, Lipoxygenase",
author = "Holtzman, {M. J.} and D. Grunberger and Hunter, {J. A.}",
note = "Funding Information: The influence of these factors on the expression of lipoxygenase and cyclooxygenase activities is also uncertain. Most efforts to modify the types of oxygenation products generated by cells have focused on substituting a substrate that results in a product with decreased biologic activity (e.g., the use of eicosapentaenoic acid) [25]. The possibility that an altered substrate matrix may also change the level of enzymatic activity is supported by the findings that lipoxygenase activity is enhanced by phospholipid [26], and cyclooxygenase activity is influenced by fatty acids [27,28]. Whether differences in membrane composition may also affect the types of oxygenation enzymes that are expressedh as not been reported, but such a mechanism might explain the heterogeneity of oxygenation activities among epithelial cells from different species. The authors wish to thank Dr. John Clements and Dr. Joanne Whitney for expert advice. M.J.H. was supported in part by a Francis S. North Fellowship. This work was also supported by NIH Program Project Grant HL-24136 and by a grant from the Council for Tobacco Research.",
year = "1986",
month = jul,
day = "18",
doi = "10.1016/0005-2760(86)90212-2",
language = "English",
volume = "877",
pages = "459--464",
journal = "Biochimica et Biophysica Acta - Lipids and Lipid Metabolism",
issn = "0005-2760",
number = "3",
}