Phosphate accumulation by the isolated renal tubule and the effects of parathyroid hormone and cyclic adenosine monophosphate

Edward M. Rosenberg; Sook Won Lee; Lewis R. Chase; Louis V. Avioli

Phosphate accumulation by the isolated renal tubule and the effects of parathyroid hormone and cyclic adenosine monophosphate

Edward M. Rosenberg
, Sook Won Lee
, Lewis R. Chase
, Louis V. Avioli

Division of General Medicine & Geriatrics

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

The effects of metabolic and transport inhibitors and PTH, cAMP, db-cAMP, and theophylline on Pi uptake by an isolated rat renal tubular preparation has been investigated. Pi uptake by this preparation was a saturable process, was O_2⁻ and sodium-dependent, was inactivated by heat-treatment, and was inhibited by dinitrophenol, iodacetate, ouabain, and a decrease in temperature from 37 ° to 4 °C. PTH and cAMP stimulated Pi uptake by energy-dependent and ouabain-inhibitable mechanisms.

Original language	English
Pages (from-to)	24-35
Number of pages	12
Journal	Journal of Laboratory and Clinical Medicine
Volume	96
Issue number	1
State	Published - Jan 1 1980

Keywords

2
4-dinitrophenyl
5 diphenyloxazole
5′-cyclic monophosphate
BSA
DNP
EGTA
KRB
Krebs-Ringer bicarbonate
PAH
PTH
Pi
TCA
TLC
adenosine 3′
bovine serum albumin
cAMP
cGMP
cyclic guanosine monophosphate
db-cAMP
dibutyryl cAMP
ethyleneglycol tetraacetic acid
p-bis-2(5-phenyloxazolyl) and 2
para-aminohippuric acid
parathyroid hormone
phosphate
trichloroacetic acid

Cite this

@article{3f89152312464c8690c49c5021d6229f,

title = "Phosphate accumulation by the isolated renal tubule and the effects of parathyroid hormone and cyclic adenosine monophosphate",

abstract = "The effects of metabolic and transport inhibitors and PTH, cAMP, db-cAMP, and theophylline on Pi uptake by an isolated rat renal tubular preparation has been investigated. Pi uptake by this preparation was a saturable process, was O2− and sodium-dependent, was inactivated by heat-treatment, and was inhibited by dinitrophenol, iodacetate, ouabain, and a decrease in temperature from 37 ° to 4 °C. PTH and cAMP stimulated Pi uptake by energy-dependent and ouabain-inhibitable mechanisms.",

keywords = "2, 4-dinitrophenyl, 5 diphenyloxazole, 5′-cyclic monophosphate, BSA, DNP, EGTA, KRB, Krebs-Ringer bicarbonate, PAH, PTH, Pi, TCA, TLC, adenosine 3′, bovine serum albumin, cAMP, cGMP, cyclic guanosine monophosphate, db-cAMP, dibutyryl cAMP, ethyleneglycol tetraacetic acid, p-bis-2(5-phenyloxazolyl) and 2, para-aminohippuric acid, parathyroid hormone, phosphate, trichloroacetic acid",

author = "Rosenberg, \{Edward M.\} and Lee, \{Sook Won\} and Chase, \{Lewis R.\} and Avioli, \{Louis V.\}",

year = "1980",

month = jan,

day = "1",

language = "English",

volume = "96",

pages = "24--35",

journal = "Journal of Laboratory and Clinical Medicine",

issn = "0022-2143",

number = "1",

}

TY - JOUR

T1 - Phosphate accumulation by the isolated renal tubule and the effects of parathyroid hormone and cyclic adenosine monophosphate

AU - Rosenberg, Edward M.

AU - Lee, Sook Won

AU - Chase, Lewis R.

AU - Avioli, Louis V.

PY - 1980/1/1

Y1 - 1980/1/1

N2 - The effects of metabolic and transport inhibitors and PTH, cAMP, db-cAMP, and theophylline on Pi uptake by an isolated rat renal tubular preparation has been investigated. Pi uptake by this preparation was a saturable process, was O2− and sodium-dependent, was inactivated by heat-treatment, and was inhibited by dinitrophenol, iodacetate, ouabain, and a decrease in temperature from 37 ° to 4 °C. PTH and cAMP stimulated Pi uptake by energy-dependent and ouabain-inhibitable mechanisms.

AB - The effects of metabolic and transport inhibitors and PTH, cAMP, db-cAMP, and theophylline on Pi uptake by an isolated rat renal tubular preparation has been investigated. Pi uptake by this preparation was a saturable process, was O2− and sodium-dependent, was inactivated by heat-treatment, and was inhibited by dinitrophenol, iodacetate, ouabain, and a decrease in temperature from 37 ° to 4 °C. PTH and cAMP stimulated Pi uptake by energy-dependent and ouabain-inhibitable mechanisms.

KW - 2

KW - 4-dinitrophenyl

KW - 5 diphenyloxazole

KW - 5′-cyclic monophosphate

KW - BSA

KW - DNP

KW - EGTA

KW - KRB

KW - Krebs-Ringer bicarbonate

KW - PAH

KW - PTH

KW - Pi

KW - TCA

KW - TLC

KW - adenosine 3′

KW - bovine serum albumin

KW - cAMP

KW - cGMP

KW - cyclic guanosine monophosphate

KW - db-cAMP

KW - dibutyryl cAMP

KW - ethyleneglycol tetraacetic acid

KW - p-bis-2(5-phenyloxazolyl) and 2

KW - para-aminohippuric acid

KW - parathyroid hormone

KW - phosphate

KW - trichloroacetic acid

UR - https://www.scopus.com/pages/publications/0018869564

M3 - Article

C2 - 6248607

AN - SCOPUS:0018869564

SN - 0022-2143

VL - 96

SP - 24

EP - 35

JO - Journal of Laboratory and Clinical Medicine

JF - Journal of Laboratory and Clinical Medicine

IS - 1

ER -

Phosphate accumulation by the isolated renal tubule and the effects of parathyroid hormone and cyclic adenosine monophosphate

Abstract

Keywords

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