TY - JOUR
T1 - Phenotypic and genomic analyses of Burkholderia stabilis clinical contamination, Switzerland
AU - Seth-Smith, Helena M.B.
AU - Casanova, Carlo
AU - Sommerstein, Rami
AU - Meinel, Dominik M.
AU - Abdelbary, Mohamed M.H.
AU - Blanc, Dominique S.
AU - Droz, Sara
AU - Führer, Urs
AU - Lienhard, Reto
AU - Lang, Claudia
AU - Dubuis, Olivier
AU - Schlegel, Matthias
AU - Widmer, Andreas
AU - Keller, Peter M.
AU - Marschall, Jonas
AU - Egli, Adrian
N1 - Funding Information:
the Burkholderia cepacia complex Multi Locus Sequence Typing website (http://pubmlst.org/bcc) developed by Keith Jolley (20), located at the University of Oxford and funded by the Wellcome Trust.
Funding Information:
We thank Clarisse Straub, Elisabeth Schultheiss, and Christine Kiessling for excellent technical assistance in performing whole-genome sequencing; Andrea Patrignani for PacBio sequencing; Doris Hohler for performing the phenotypic and antimicrobial testing; and Peter Vandamme for advice on species definition. Assemblies were performed at sciCORE (http://scicore.unibas.ch) scientific computing facility at the University of Basel. A.E. received a research grant from the Swiss National Science Foundation (Ambizione grant no. PZ00P3_154709/1). We used the Burkholderia cepacia complex Multi Locus Sequence Typing website (http://pubmlst.org/bcc) developed by Keith Jolley (20), located at the University of Oxford and funded by the Wellcome Trust.
Funding Information:
A.E. received a research grant from the Swiss National Science Foundation (Ambizione grant no. PZ00P3_154709/1). We used
Publisher Copyright:
© 2019, Centers for Disease Control and Prevention (CDC). All rights reserved.
PY - 2019/6
Y1 - 2019/6
N2 - A recent hospital outbreak related to premoistened gloves used to wash patients exposed the difficulties of defining Burkholderia species in clinical settings. The outbreak strain displayed key B. stabilis phenotypes, including the inability to grow at 42°C; we used whole-genome sequencing to confirm the pathogen was B. stabilis. The outbreak strain genome comprises 3 chromosomes and a plasmid, sharing an average nucleotide identity of 98.4% with B. stabilis ATCC27515 BAA-67, but with 13% novel coding sequences. The genome lacks identifiable virulence factors and has no apparent increase in encoded antimicrobial drug resistance, few insertion sequences, and few pseudogenes, suggesting this outbreak was an opportunistic infection by an environmental strain not adapted to human pathogenicity. The diversity among outbreak isolates (22 from patients and 16 from washing gloves) is only 6 single-nucleotide polymorphisms, although the genome remains plastic, with large elements stochastically lost from outbreak isolates.
AB - A recent hospital outbreak related to premoistened gloves used to wash patients exposed the difficulties of defining Burkholderia species in clinical settings. The outbreak strain displayed key B. stabilis phenotypes, including the inability to grow at 42°C; we used whole-genome sequencing to confirm the pathogen was B. stabilis. The outbreak strain genome comprises 3 chromosomes and a plasmid, sharing an average nucleotide identity of 98.4% with B. stabilis ATCC27515 BAA-67, but with 13% novel coding sequences. The genome lacks identifiable virulence factors and has no apparent increase in encoded antimicrobial drug resistance, few insertion sequences, and few pseudogenes, suggesting this outbreak was an opportunistic infection by an environmental strain not adapted to human pathogenicity. The diversity among outbreak isolates (22 from patients and 16 from washing gloves) is only 6 single-nucleotide polymorphisms, although the genome remains plastic, with large elements stochastically lost from outbreak isolates.
UR - http://www.scopus.com/inward/record.url?scp=85066946235&partnerID=8YFLogxK
U2 - 10.3201/eid2506.172119
DO - 10.3201/eid2506.172119
M3 - Article
C2 - 31107229
AN - SCOPUS:85066946235
VL - 25
SP - 1084
EP - 1092
JO - Emerging Infectious Diseases
JF - Emerging Infectious Diseases
SN - 1080-6040
IS - 6
ER -