Phagocytosis by human monocytes of unopsonized particulate activators of the human alternative complement pathway: Induction by cytokine

B. Chakravarti, R. D. Schreiber, H. J. Muller-Eberhard

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Monocytes isolated from human blood by centrifugal elutriation exhibited little ability to ingest rabit erythrocytes (E(R)), zymosan particles, or desialated sheep erythrocytes. In contrast, 85 to 95% of these cells rosetted with C3b- or C3bi-bearing sheep erythrocytes (E(S)) or ingested IgG-coated E(S). Preincubation of the monocytes with human lymphocytes increased their ability to ingest E(R). The E(R) phagocytosis-inducing activity was contained in the 105,000 x G supernatant of lymphocyte lysates. These supernatants increased the percentage of ingesting monocytes from 5 to 15% to 80% within 60 min. The soluble factor was found to be relatively heat stable, inactivated by trypsin, and distinct from IFN-γ. Its m.w. is less than 13,000. It was present in B and T lymphocytes and also in U937 cells. These results suggest that the ability of human monocytes to ingest nonopsonized particulate activators of the alternative complement pathway is a cytokine-inducible property and that the effect of the cytokine on complement receptor- or Fc receptor-dependent adherence or ingestion of opsonized particles is minor.

Original languageEnglish
Pages (from-to)880-886
Number of pages7
JournalJournal of Immunology
Volume137
Issue number3
StatePublished - Jan 1 1986
Externally publishedYes

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