Phage f1 mRNA processing in Escherichia coli: search for the upstream products of endonuclease cleavage, requirement for the product of the altered mRNA stability (ams) locus

R. J. Kokoska, K. J. Blumer, D. A. Steege

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

In Escherichia coli infected with the filamentous phage f1, a number of the polycistronic phage mRNA species are generated through post-transcriptional processing by host nuclease activity. In this paper we review experimental evidence assessing whether known RNases are involved in mediating these processing events, and we use S1 nuclease mapping methods to visualize putative upstream products of endonuclease cleavage. By examining f1 processing in a phage-infected host bearing a temperature-sensitive allele of the altered message stability locus (ams), we show that production of the major processed species requires a component of the host cell which functions in the messenger RNA decay process.

Original languageEnglish
Pages (from-to)803-811
Number of pages9
JournalBiochimie
Volume72
Issue number11
DOIs
StatePublished - Nov 1990

Keywords

  • altered message stability
  • endonuclease cleavage
  • mRNA processing

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