TY - JOUR
T1 - Phage displayed HBV core antigen with immunogenic activity
AU - Bahadir, Aylin Ozdemir
AU - Balcioglu, Bertan Koray
AU - Uzyol, Kamil Serkan
AU - Hatipoglu, Ibrahim
AU - Sogut, Ibrahim
AU - Basalp, Aynur
AU - Erdag, Berrin
PY - 2011/12
Y1 - 2011/12
N2 - Hepatitis B is a major public health problem worldwide, which may lead to chronic liver diseases such as cirrhosis and hepatocellular carcinoma. The hepatitis B core antigen (HBcAg) is one of the major viral proteins, which forms the inner core of hepatitis B virus (HBV) particles. In this study, filamentous bacteriophage M13 was genetically modified to display the polypeptides of HBcAg in order to develop an alternative carrier system. HBcAg gene was inserted into the minor coat protein (pIII) gene of M13, and HBcAg was expressed on the phage surface as a whole protein. Antigenicity and immunogenicity of HBcAg were tested by immunizing BALB/c mice three times with HBcAg-displaying recombinant phages. After successful immunization, one of the mice with high antibody titer to HBcAg was selected for fusion, and four monoclonal antibodies specific for HBcAg were developed. This result showed that HBcAg-displaying recombinant bacteriophages are immunogenic and can potentially be used for the development of monoclonal antibodies.
AB - Hepatitis B is a major public health problem worldwide, which may lead to chronic liver diseases such as cirrhosis and hepatocellular carcinoma. The hepatitis B core antigen (HBcAg) is one of the major viral proteins, which forms the inner core of hepatitis B virus (HBV) particles. In this study, filamentous bacteriophage M13 was genetically modified to display the polypeptides of HBcAg in order to develop an alternative carrier system. HBcAg gene was inserted into the minor coat protein (pIII) gene of M13, and HBcAg was expressed on the phage surface as a whole protein. Antigenicity and immunogenicity of HBcAg were tested by immunizing BALB/c mice three times with HBcAg-displaying recombinant phages. After successful immunization, one of the mice with high antibody titer to HBcAg was selected for fusion, and four monoclonal antibodies specific for HBcAg were developed. This result showed that HBcAg-displaying recombinant bacteriophages are immunogenic and can potentially be used for the development of monoclonal antibodies.
KW - ELISA
KW - HBcAg
KW - Immune response
KW - M13 phage
KW - Monoclonal antibody
KW - Phage display
UR - http://www.scopus.com/inward/record.url?scp=81955160714&partnerID=8YFLogxK
U2 - 10.1007/s12010-011-9365-1
DO - 10.1007/s12010-011-9365-1
M3 - Article
C2 - 21915589
AN - SCOPUS:81955160714
SN - 0273-2289
VL - 165
SP - 1437
EP - 1447
JO - Applied Biochemistry and Biotechnology
JF - Applied Biochemistry and Biotechnology
IS - 7-8
ER -