PET/CT imaging with an 18F-labeled galactodendritic unit in a Galectin-1–overexpressing orthotopic bladder cancer model

Patricia M.R. Pereira, Sheryl Roberts, Flávio Figueira, João P.C. Tomé, Thomas Reiner, Jason S. Lewis

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Galectins are carbohydrate-binding proteins overexpressed in bladder cancer (BCa) cells. Dendritic galactose moieties have a high affinity for galectin-expressing tumor cells. We radiolabeled a dendritic galactose carbohydrate with 18F (18F-labeled galactodendritic unit 4) and examined its potential in imaging urothelial malignancies. Methods: The 18F-labeled first-generation galactodendritic unit 4 was obtained from its tosylate precursor. We conducted in vivo studies in a galectin-expressing UMUC3 orthotopic BCa model to determine the ability of 18F-labeled galactodendritic unit 4 to image BCa. Results: Intravesical administration of 18F-labeled galactodendritic unit 4 allowed specific accumulation of the carbohydrate radiotracer in galectin-1–overexpressing UMUC3 orthotopic tumors when imaged with PET. The 18F-labeled galactodendritic unit 4 was not found to accumulate in nontumor murine bladders. Conclusion: The 18F-labeled galactodendritic unit 4 and similar analogs may be clinically relevant and exploitable for PET imaging of galectin-1–overexpressing bladder tumors.

Original languageEnglish
Pages (from-to)1369-1375
Number of pages7
JournalJournal of Nuclear Medicine
Volume61
Issue number9
DOIs
StatePublished - Sep 1 2020

Keywords

  • Bladder cancer
  • Dendritic carbohydrate
  • F-radiochemistry
  • Galectins
  • PET/CT imaging

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