PET Imaging Study of S1PR1 Expression in a Rat Model of Multiple Sclerosis

Hui Liu; Hongjun Jin; Xuyi Yue; Zonghua Luo; Chunling Liu; Adam J. Rosenberg; Zhude Tu

doi:10.1007/s11307-016-0944-y

PET Imaging Study of S1PR1 Expression in a Rat Model of Multiple Sclerosis

Hui Liu, Hongjun Jin, Xuyi Yue, Zonghua Luo, Chunling Liu, Adam J. Rosenberg, Zhude Tu

Research output: Contribution to journal › Article › peer-review

40 Scopus citations

Abstract

Purpose: Upregulation of sphingosine-1-phosphate receptor 1 (S1PR1) expression in multiple sclerosis (MS) lesions is associated with neuroinflammatory response. This study investigated the correlation between neuroinflammation and S1PR1 expression in the spinal cord of an experimental autoimmune encephalomyelitis (EAE) rat model of MS, using the S1PR1 positron emission tomography (PET) radiotracer [¹¹C]TZ3321. Procedures: MicroPET imaging studies of [¹¹C]TZ3321 were performed to measure uptake of [¹¹C]TZ3321 in the spinal cord of EAE rats. Immunohistochemical staining was performed to confirm the overexpression of S1PR1 and other inflammatory biomarkers. Results: MicroPET imaging demonstrated a 20–30 % increase in [¹¹C]TZ3321 uptake in the lumbar spinal cord of EAE rats versus sham controls at 35–60 min post injection. The increased uptake of [¹¹C]TZ3321 was correlated with the overexpression of S1PR1 in the lumbar spinal cord of EAE rats that was confirmed by immunohistochemical staining. Upregulated S1PR1 expression was associated with glial cell activation and immune cell infiltration. Conclusions: MicroPET imaging modality with a specific radioligand [¹¹C]TZ3321 is able to assess the expression of S1PR1 in EAE rat lumbar spinal cord. This may provide a new approach to the assessment of neuroinflammatory response in MS and other inflammatory diseases.

Original language	English
Pages (from-to)	724-732
Number of pages	9
Journal	Molecular Imaging and Biology
Volume	18
Issue number	5
DOIs	https://doi.org/10.1007/s11307-016-0944-y
State	Published - Oct 1 2016

Keywords

Experimental autoimmune encephalomyelitis
Multiple sclerosis
Neuroinflammation
PET imaging
Radiotracer
Sphingosine-1-phosphate receptor 1

Access to Document

10.1007/s11307-016-0944-y

Cite this

@article{861b074fd3914e55ba348951ddaf4f04,

title = "PET Imaging Study of S1PR1 Expression in a Rat Model of Multiple Sclerosis",

abstract = "Purpose: Upregulation of sphingosine-1-phosphate receptor 1 (S1PR1) expression in multiple sclerosis (MS) lesions is associated with neuroinflammatory response. This study investigated the correlation between neuroinflammation and S1PR1 expression in the spinal cord of an experimental autoimmune encephalomyelitis (EAE) rat model of MS, using the S1PR1 positron emission tomography (PET) radiotracer [11C]TZ3321. Procedures: MicroPET imaging studies of [11C]TZ3321 were performed to measure uptake of [11C]TZ3321 in the spinal cord of EAE rats. Immunohistochemical staining was performed to confirm the overexpression of S1PR1 and other inflammatory biomarkers. Results: MicroPET imaging demonstrated a 20–30 % increase in [11C]TZ3321 uptake in the lumbar spinal cord of EAE rats versus sham controls at 35–60 min post injection. The increased uptake of [11C]TZ3321 was correlated with the overexpression of S1PR1 in the lumbar spinal cord of EAE rats that was confirmed by immunohistochemical staining. Upregulated S1PR1 expression was associated with glial cell activation and immune cell infiltration. Conclusions: MicroPET imaging modality with a specific radioligand [11C]TZ3321 is able to assess the expression of S1PR1 in EAE rat lumbar spinal cord. This may provide a new approach to the assessment of neuroinflammatory response in MS and other inflammatory diseases.",

keywords = "Experimental autoimmune encephalomyelitis, Multiple sclerosis, Neuroinflammation, PET imaging, Radiotracer, Sphingosine-1-phosphate receptor 1",

author = "Hui Liu and Hongjun Jin and Xuyi Yue and Zonghua Luo and Chunling Liu and Rosenberg, {Adam J.} and Zhude Tu",

note = "Publisher Copyright: {\textcopyright} 2016, World Molecular Imaging Society.",

year = "2016",

month = oct,

day = "1",

doi = "10.1007/s11307-016-0944-y",

language = "English",

volume = "18",

pages = "724--732",

journal = "Molecular Imaging and Biology",

issn = "1536-1632",

number = "5",

}

TY - JOUR

T1 - PET Imaging Study of S1PR1 Expression in a Rat Model of Multiple Sclerosis

AU - Liu, Hui

AU - Jin, Hongjun

AU - Yue, Xuyi

AU - Luo, Zonghua

AU - Liu, Chunling

AU - Rosenberg, Adam J.

AU - Tu, Zhude

PY - 2016/10/1

Y1 - 2016/10/1

N2 - Purpose: Upregulation of sphingosine-1-phosphate receptor 1 (S1PR1) expression in multiple sclerosis (MS) lesions is associated with neuroinflammatory response. This study investigated the correlation between neuroinflammation and S1PR1 expression in the spinal cord of an experimental autoimmune encephalomyelitis (EAE) rat model of MS, using the S1PR1 positron emission tomography (PET) radiotracer [11C]TZ3321. Procedures: MicroPET imaging studies of [11C]TZ3321 were performed to measure uptake of [11C]TZ3321 in the spinal cord of EAE rats. Immunohistochemical staining was performed to confirm the overexpression of S1PR1 and other inflammatory biomarkers. Results: MicroPET imaging demonstrated a 20–30 % increase in [11C]TZ3321 uptake in the lumbar spinal cord of EAE rats versus sham controls at 35–60 min post injection. The increased uptake of [11C]TZ3321 was correlated with the overexpression of S1PR1 in the lumbar spinal cord of EAE rats that was confirmed by immunohistochemical staining. Upregulated S1PR1 expression was associated with glial cell activation and immune cell infiltration. Conclusions: MicroPET imaging modality with a specific radioligand [11C]TZ3321 is able to assess the expression of S1PR1 in EAE rat lumbar spinal cord. This may provide a new approach to the assessment of neuroinflammatory response in MS and other inflammatory diseases.

AB - Purpose: Upregulation of sphingosine-1-phosphate receptor 1 (S1PR1) expression in multiple sclerosis (MS) lesions is associated with neuroinflammatory response. This study investigated the correlation between neuroinflammation and S1PR1 expression in the spinal cord of an experimental autoimmune encephalomyelitis (EAE) rat model of MS, using the S1PR1 positron emission tomography (PET) radiotracer [11C]TZ3321. Procedures: MicroPET imaging studies of [11C]TZ3321 were performed to measure uptake of [11C]TZ3321 in the spinal cord of EAE rats. Immunohistochemical staining was performed to confirm the overexpression of S1PR1 and other inflammatory biomarkers. Results: MicroPET imaging demonstrated a 20–30 % increase in [11C]TZ3321 uptake in the lumbar spinal cord of EAE rats versus sham controls at 35–60 min post injection. The increased uptake of [11C]TZ3321 was correlated with the overexpression of S1PR1 in the lumbar spinal cord of EAE rats that was confirmed by immunohistochemical staining. Upregulated S1PR1 expression was associated with glial cell activation and immune cell infiltration. Conclusions: MicroPET imaging modality with a specific radioligand [11C]TZ3321 is able to assess the expression of S1PR1 in EAE rat lumbar spinal cord. This may provide a new approach to the assessment of neuroinflammatory response in MS and other inflammatory diseases.

KW - Experimental autoimmune encephalomyelitis

KW - Multiple sclerosis

KW - Neuroinflammation

KW - PET imaging

KW - Radiotracer

KW - Sphingosine-1-phosphate receptor 1

UR - http://www.scopus.com/inward/record.url?scp=84961122240&partnerID=8YFLogxK

U2 - 10.1007/s11307-016-0944-y

DO - 10.1007/s11307-016-0944-y

M3 - Article

C2 - 26975859

AN - SCOPUS:84961122240

SN - 1536-1632

VL - 18

SP - 724

EP - 732

JO - Molecular Imaging and Biology

JF - Molecular Imaging and Biology

IS - 5

ER -

PET Imaging Study of S1PR1 Expression in a Rat Model of Multiple Sclerosis

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this