Perturbation of nuclear architecture by long-distance chromosome interactions

Abby F. Dernburg, Karl W. Broman, Jennifer C. Fung, Wallace F. Marshall, Jennifer Philips, David A. Agard, John W. Sedat

Research output: Contribution to journalArticlepeer-review

347 Scopus citations

Abstract

Position-effect variegation (PEV) describes the stochastic transcriptional silencing of a gene positioned adjacent to heterochromatin. Using FISH, we have tested whether variegated expression of the eye-color gene brown in Drosophila is influenced by its nuclear localization. In embryonic nuclei, a heterochromatic insertion at the brown locus is always spatially isolated from other heterochromatin. However, during larval development this insertion physically associates with other heterochromatic regions on the same chromosome in a stochastic manner. These observations indicate that the brown gene is silenced by specific contact with centromeric heterochromatin. Moreover, they provide direct evidence for long-range chromosome interactions and their impact on three-dimensional nuclear architecture, while providing a cohesive explanation for the phenomenon of PEV.

Original languageEnglish
Pages (from-to)745-759
Number of pages15
JournalCell
Volume85
Issue number5
DOIs
StatePublished - May 31 1996
Externally publishedYes

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