TY - JOUR
T1 - Perturbation of nuclear architecture by long-distance chromosome interactions
AU - Dernburg, Abby F.
AU - Broman, Karl W.
AU - Fung, Jennifer C.
AU - Marshall, Wallace F.
AU - Philips, Jennifer
AU - Agard, David A.
AU - Sedat, John W.
N1 - Funding Information:
Analysis and display of our images were made possible through the tremendous efforts of Hans Chen, Paul Chan, and Diana Hughes. Advice on statistical analysis from Terry Speed, Peter Bacchetti, and Ray Sachs was very much appreciated. We thank Jeremy Minshull, Jeff Sekelsky, Scott Hawley, Elizabeth Blackburn, and Jim Haber for critical reading of the manuscript. This work was supported by the Howard Hughes Medical Institute Predoctoral Fellowships to A. F. D. and W. F. M. and by a National Institutes of Health grant to J. W. S.
PY - 1996/5/31
Y1 - 1996/5/31
N2 - Position-effect variegation (PEV) describes the stochastic transcriptional silencing of a gene positioned adjacent to heterochromatin. Using FISH, we have tested whether variegated expression of the eye-color gene brown in Drosophila is influenced by its nuclear localization. In embryonic nuclei, a heterochromatic insertion at the brown locus is always spatially isolated from other heterochromatin. However, during larval development this insertion physically associates with other heterochromatic regions on the same chromosome in a stochastic manner. These observations indicate that the brown gene is silenced by specific contact with centromeric heterochromatin. Moreover, they provide direct evidence for long-range chromosome interactions and their impact on three-dimensional nuclear architecture, while providing a cohesive explanation for the phenomenon of PEV.
AB - Position-effect variegation (PEV) describes the stochastic transcriptional silencing of a gene positioned adjacent to heterochromatin. Using FISH, we have tested whether variegated expression of the eye-color gene brown in Drosophila is influenced by its nuclear localization. In embryonic nuclei, a heterochromatic insertion at the brown locus is always spatially isolated from other heterochromatin. However, during larval development this insertion physically associates with other heterochromatic regions on the same chromosome in a stochastic manner. These observations indicate that the brown gene is silenced by specific contact with centromeric heterochromatin. Moreover, they provide direct evidence for long-range chromosome interactions and their impact on three-dimensional nuclear architecture, while providing a cohesive explanation for the phenomenon of PEV.
UR - http://www.scopus.com/inward/record.url?scp=0029943141&partnerID=8YFLogxK
U2 - 10.1016/S0092-8674(00)81240-4
DO - 10.1016/S0092-8674(00)81240-4
M3 - Article
C2 - 8646782
AN - SCOPUS:0029943141
VL - 85
SP - 745
EP - 759
JO - Cell
JF - Cell
SN - 0092-8674
IS - 5
ER -