Personalized Single-Cell Proteogenomics to Distinguish Acute Myeloid Leukemia from Non-Malignant Clonal Hematopoiesis

Laura W. Dillon, Jack Ghannam, Chidera Nosiri, Gege Gui, Meghali Goswami, Katherine R. Calvo, Katherine E. Lindblad, Karolyn A. Oetjen, Matthew D. Wilkerson, Anthony R. Soltis, Gauthaman Sukumar, Clifton L. Dalgard, Julie Thompson, Janet Valdez, Christin B. DeStefano, Catherine Lai, Adam Sciambi, Robert Durruthy-Durruthy, Aaron Llanso, Saurabh GulatiShu Wang, Aik Ooi, Pradeep K. Dagur, J. Philip McCoy, Patrick Burr, Yuesheng Li, Christopher S. Hourigan

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Genetic mutations associated with acute myeloid leukemia (AML) also occur in age-related clonal hematopoiesis, often in the same individual. This makes confident assignment of detected variants to malignancy challenging. The issue is particularly crucial for AML post-treatment measurable residual disease monitoring, where results can be discordant between genetic sequencing and flow cytometry. We show here, that it is possible to distinguish AML from clonal hematopoiesis and to resolve the immunophenotypic identity of clonal architecture. To achieve this, we first design patient-specific DNA probes based on patient's whole-genome sequencing, and then use them for patient-personalized single-cell DNA sequencing with simultaneous single-cell antibody-oligonucleotide sequencing. Examples illustrate AML arising from DNMT3A and TET2 mutated clones as well as independently. The ability to personalize single-cell proteogenomic assessment for individual patients based on leukemia-specific genomic features has implications for ongoing AML precision medicine efforts.

Original languageEnglish
Pages (from-to)319-325
Number of pages7
JournalCancer discovery
Volume2
Issue number4
DOIs
StatePublished - Jul 1 2021

Keywords

  • acute myeloid leukemia
  • clonal hematopoiesis
  • personalized medicine
  • proteogenomics
  • single-cell DNA sequencing

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