TY - JOUR
T1 - Peripheral-type benzodiazepine receptors in endocrine organs
T2 - Autoradiographic localization in rat pituitary, adrenal, and testis
AU - De Souza, Errol B.
AU - Anholt, Robert R.H.
AU - Murphy, Kenneth M.M.
AU - Snyder, Solomon H.
AU - Kuhar, Michael J.
PY - 1985/1/1
Y1 - 1985/1/1
N2 - We have used [3H]Ro5-4864, a ligand selective for peripheral-type benzodiazepine receptors, to identify and localize peripheral-type benzodiazepine receptors in endocrine organs. Autoradiographic studies reveal an uniform distribution of [3H]Ro5-4864 binding sites within the anterior, intermediate, and posterior lobes of the pituitary gland, with highest concentrations present in the posterior pituitary. In rat adrenal gland, specific binding sites for [3H]Ro5-4864 are found only in the adrenal cortex, with highest density in the zona glomerulosa and significantly lower concentrations in the zona fasciculata and zona reticularis. [3H]Ro5-4864-associated silver grains in the testis are intensely localized over the interstitial tissue; low concentrations of silver grains are present over the epithelium of the seminiferous tubules but are absent from the tubular lumen. These studies demonstrate a differential and discrete localization of peripheral-type benzodiazepine receptors in rat pituitary, adrenal, and testis.
AB - We have used [3H]Ro5-4864, a ligand selective for peripheral-type benzodiazepine receptors, to identify and localize peripheral-type benzodiazepine receptors in endocrine organs. Autoradiographic studies reveal an uniform distribution of [3H]Ro5-4864 binding sites within the anterior, intermediate, and posterior lobes of the pituitary gland, with highest concentrations present in the posterior pituitary. In rat adrenal gland, specific binding sites for [3H]Ro5-4864 are found only in the adrenal cortex, with highest density in the zona glomerulosa and significantly lower concentrations in the zona fasciculata and zona reticularis. [3H]Ro5-4864-associated silver grains in the testis are intensely localized over the interstitial tissue; low concentrations of silver grains are present over the epithelium of the seminiferous tubules but are absent from the tubular lumen. These studies demonstrate a differential and discrete localization of peripheral-type benzodiazepine receptors in rat pituitary, adrenal, and testis.
UR - http://www.scopus.com/inward/record.url?scp=0021944108&partnerID=8YFLogxK
U2 - 10.1210/endo-116-2-567
DO - 10.1210/endo-116-2-567
M3 - Article
C2 - 2981667
AN - SCOPUS:0021944108
VL - 116
SP - 567
EP - 573
JO - Endocrinology
JF - Endocrinology
SN - 0013-7227
IS - 2
ER -